Study On Effects Of T-PA And PAI-1 On The Macro-vascular Complications In Type 2 Diabetes Mellitus

Objective: To identify the importance of abnormal fibrinolytic system especially the content change of tissue-plasminogen activator(t-PA) and plasminogen activator inhibitor-1(PAI-1)in the process of macro-vascular complications in type 2 diabetes mellitus and provide theoretical evidences to suppress or delay the occurrence and development of macro-vascular complications in type 2 diabetes mellitus effectually for clinical research.Methods: Paired design is applied in this research and 80 cases are selected including 44 men and 36 women. Those two patients are paired with same gender and basically equivalent age, blood sugar, blood pressure and blood-lipid but opposite result of intima media thickness( IMT), so 80 cases are divided into two average groups. Type 2 diabetes mellitus with astherosclerosis (AS-T2DM) group has 40 cases, 22 men and 18 women. So does type 2 diabetes mellitus without astherosclerosis (nonAS-T2DM) group. There are no significant differences in age,blood sugar, blood pressure and blood-lipid between the two groups verified by paired samples T test. (t=1.785,1.779,1.568,0.479 and P=0.214,0.225,0.476,0.622 respectively)The contents of t-PA and PAI-1 in the two groups are measured quantitatively by double-antibody sandwich ELISA at same time. Each sample is measured 3 times repeatedly to ensure the accuracy of the measurement and the mean is used for statistics. The differences of t-PA and PAI-1 contents between the two groups are compared by paired samples T test.Result: Compared with nonAS-T2DM group, the content of t-PA reduced significantly (t=2.384,P=0.021) while PAI-1 increased significantly (t=2.563,P=0.015) in AS-T2DM group.Conclusion: The reduce of t-PA content and increase of PAI-1 content have played some role in the occurrence and development of macro-vascular complications in type 2 diabetes mellitus....