Studies On Inducing Apoptosis Of The Combination Of HCPT And Grub For MGC-803 Human Gastric Cancer Cell Line In Vitro

Objective: To investigate the anti-proliferation and inducing apoptosis of the combination of HCPT and grub for MGC-803gastric cancer cell line in vitro.Methods: Using the MTT assay, cell growth curve and colonogenic assay was examined the inhibition of cell's growth and the sensitivity of the two drugs for MGC-803 gastric cancer cells; using HE stain, scanning electron microscope and acridine orange(AO)/ethidium bromide(EB) fluorescence stain, the inhibiting proliferation and inducing apoptosis of for MGC-803 gastric cancer cells were examined. The expression changes of relating-apoptosis gene proteins (bcl-2, ki-67, bax) were detected by immunohistochemistry (S-P method) before and after treating with the medicine to explore the possible mechanisms of inhibiting proliferations and inducing apoptosis for MGC-803 cells by treatingwith the combination of HCPT and grub medicine in vitro.Results: The combination of HCPT and grub significantlyinhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration andtreating times. After treating 24 hours on the gastric cancer cells with the two drugs, morphologic changes of apoptosis with chromatinmargination, karyopyknosis, karyorrhexis, apoptotic body were found by the light and electron microscope. It was showed by using acridine orange (AO) / ethidium bromide (EB) fluorescence staining that MGC-803 gastric cancer cells after treated by the combination ofHCPT and grub medicine were not only necrosis but also apoptosis,but the apoptosis was as the dominant way. The bcl-2, ki-67 and bax of relating-apoptosis genes was significantly changed before and aftertreating with the combination of HCPT and grub (P<0.001) .Conclusions: The combination of HCPT and grub has significantinhibition of proliferation and inducing apoptosis for MGC-803 gastric cancer cells in vitro and its possible mechanism of inducing apoptosis for tumor cells might be related with the expression changes of the bcl-2, ki-67 and bax of relating-apoptosis genes. This experiment results will provide theoretical basis for clinical applying and further research of the HCPT.