| Abnormal scar includes hypertrophic scar (HS) and keloid. They are proliferative result from wound epithelialization secondary to skin traumatic injury. Hypertrophic scar and keloid present part-proliferation, pruritus, function hinder and impact beautiful. Both are characterized by excessive proliferation of fibroblasts and extracellular matrix (ECM), especially accumulation of collagen and their mechanism of formation are not well understood. Therefore, prevention and treatment of the abnormal scar are very difficult. At present, the methods to treatment of abnormal scar are dissatisfactory and the rate of recur is higher. So, it is critically important to investigate the pathogenesis of abnormal scar.With the further researching, it found that abnormal scar is result from the deposistion of collagen during the process of wound healing. It will be helpful to announce the machanism of abnormal scar by investigating the zymin that can degrade collagen and its correlative factors, which provide new methor to prevent and therapy abnormal scar. Matrix metalloproteinases-1 (MMP-1) is an improtant zymin to degrade collagen, whose expression and activity are regulated by many factors in which growth factors and tissue inhibitor of metalloproteinases (TIMPs) are very important. TIMP-1 can inhibit the activity of MMP-1, and then prevent of the degrade collagen. ?Platelet-derived growth factor (PDGF), a cytodine with multifunction, can stimulate fibroblasts producing collagen. In many researches, it is believed that PDGF is correlated with scar formation.Proliferating cell nuclear antigen (PCNA) is a nuclear and nucleoar protein, which play an important role during the process of copying DNA. So it is tightly associated with somatic cellproliferation. At present, the research of the degradation of collagen is rare.The views about the expression of MMP-1, TIMP-1 are not agree with each other , and the correlation with the expression of PCNA and PDGF are not clear .In the present study by using immunnohistochemistry method, we detected the expression of MMP-1, TIMP-1, PDGF and PCNA in 58 samples of abnormal scars to investigate the relationship between the expression of these proteins and the formation of abnormal scar. It will provide new aims for prevent and therapy abnormal scar. Material and method:The tissue of abnormal scar was obtained from 58 patient who were underwent surgery; Scar tissue were collected from face, neck ,chest, shoulder abdomen and limbs. There were 33 males and 25 femals.The age of patients ranged from 4 to 67 years old (mean : 25.22 years old ). Abnormal scar were divided into a few groups according to their clinical features: 1. HS: Duration: 6-24 monthes,hypertrophic scar remain with the boundaries of the original wound. 2. Keloid : Duration: 6-24 monthes, keloid extend beyond the original area of skin injury. Sixteen cases of normal skin collected from patients with abnormal scar and Sixteen cases of normotrophic scar which are charactered by no redness, pruritus, pain, and flattened with surrounded skin were used as control. Normotrophic scars were collected from face, neck and limbs. An immunohistochemical SABC method was used to detect the expression of MMP-1, TIMP-1, PDGF and PCNA in abnormal scar and the two control groups, and the correlation among the expression of these proteins were systematically studied.The statistical analysis was executed by spss software. P values less than 0.05 are considered significant. Result:1. The expression of MMP-1,TIMP-1,PDGFand PCNA in normotrophic scars and normal skins well all negative.2. The expression of MMP-1,TIMP-1, PDGF were localized in the cytoplasm .The positive rate of MMP-1 ,TIMP-1 and PDGF in abnormal scars were 62.07% (36/58), 63.79% (37/58) and 55.17% (32/58) respectively .There were significant difference in the expression of MMP-1, TIMP-1, PDGF between abnormal scar group and the two control groups .(p<0.05)3. The expression of PCNA was localized in the nuclei.In abnormal scar, normtrophic scar and n...
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