| Objective to investigate the role of platelet-activating factor(PAF) in patients with glomerulopathy, and the relation between PAF and tumor necrosis factor-Alpha(TNF-a) or interleukin-8(IL-8). To research for the relationship between PAF and thromboxane A2(TXA2). Methods The plasma level of PAF was determined by bioassay. The serum level of TNF-a and IL-8 were tested by double antibody sandwich ELISA in 32 patients with glomerulopathy and 16 healthy controls. Meanwhile, the levels of creatinine and 24h urinary protein excretion were measured. The plasma levels of thromboxane B2(TXB2) and 6-Keto-prostaglandin F1a(6-Keto-PGF1a) in 12 patients with primary nephrotic syndrome were tested by RIA. Results The patients were divided into three groups: chronic glomerulonephritis, primary nephritic syndrome, the others groups. The levels of PAF, TNF-a, IL-8 in the chronic glomerulonephritis group, primary nephrotic syndrome group and the others group were higher than that of normal control group (p<0.05). The PAF levels in the patients of the three groups were positively correlated with the TNF-a, r=0.587, 0.872, 0.702; and positively correlated with the IL-8 levels, too. r=0.612, 0.683, 0.787. The PAF levels were not correlated with the serum creatinine levels in the glomerulonephritis group; in the primary nephrotic syndrome group, the PAF levels were positively correlated with that of 24h urinary protein excretion(r=0.813, p<0.01). The levels of plasma PAF in 12 patients with primary nephrotic syndrome werepositively correlated with that of TXB2 (r=0.724, P<0.01). But were not correlated with 6-Keto-PGF1a. [Conclusion] PAF is an important mediator of injury in glomerulopathy, the communication network of PAF with TNF or IL-8 plays an important role in the pathogenesis of glomerulopathy. In primary nephrotic syndrome, the level of TXB2 increased with a significant correlation between PAF and TXB2. The result suggests that the renal injury induced by PAF could be involved with TXA2.
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