Comparison Of Biological Features Between Chitosan And Hyaluronate Sodium

Objective: To compare the effectiveness of chitosan and hyaluronate sodium in the following three aspects: the effect of bacteriostasis, preventing intestinal adhesion of rats, The effects on the proliferation of epidermal keratinocyte cells, fibroblast cells and vascular endothelial cells in vitro.Methods: 1. Each of the five bacteria, Proteus mirabilis, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus, was cultivated of broth culture. Ploidy diluted dosage of high relative molecular weight chitosan, low relative molecular weight chitosan and hyaluronate sodium were added respectively in the broth culture. All the tubes were cultivated for 18 hours on 37 with homeothermia. Then the growth of bacteria was observed 2. Forty-five rats were randomly divided into A, B and C group. During operation, chitosan, sodium hyalurate or normal saline was applied on injured part of ileum respectively. Biopsy was taken on the 14th postoperative day. 3. The cultured epidermal keratinocyte cells, fibroblast cells and vascular endothelial cells were treated with chitosan and sodium hyaluronate, cell proliferation was detected by MTT assay, the DNA content of the cells were measured by flow cytometry (FCM) .Results: 1. The minimal inhibitory concentration(MIC) of high relative molecular weight chitosan was: Proteus mirabilis 0.031%, Escherichia coli 0.063%, Candida albicans 0.063%, Pseudomonas aeruginosa 0.063%, Staphylococcus aureus 0.063%; the MIC of low relative molecular weight chitosan was: Proteus mirabilis 0.125%, Escherichia coli 0.25%, Candida albicans 0.25%, Pseudomonas aeruginosa, 0.25%, Staphylococcus aureus 0.125%; there were significantly more bacteria in the group of hyaluronate sodium. 2. The strength and number of adhesion of group A and B were significantly reduced. Mild inflammatory changes and less fibrous proliferation could be found in group A and B by microscopy which was opposite to group C. In group A, mesotheliums rehabilitated better than that of group B, and the proliferation offibroblast were not significant under electron microscope. In group B, fibroblast excreted collagen actively. 3. Chitosan(0 Img/ml) significantly (P<0.05) increased the proliferation of EKCs relative to the control group and decreased Gl phase cell rate, however, there is no difference between sodium hyaluronate and the control group in terms of the proliferation of EKCs. Chitosan(0.1mg/ml) showed a significant (P<0.05) reduction in the proliferation of fibroblast cells relative to control and increased Gl phase cell rate, while sodium Hyaluronate inhibited the proliferation of fibroblast cells at concentration of Img/ml. Chitosan( 0.lmg/ml) significantly (P<0.05) increased the proliferation of EV304 cells relative to control and decreased Gl phase cell rate, however, sodium hyaluronate inhibited the proliferation of EV304 cells and increased Gl phase cell rate.Conclusion: 1. Hyaluronate sodium shows no bacteriostasis, while chitosan shows bacteriostasis on broad spectrum and high relative molecular weight chitosan has stronger effect 2. Both chitosan and sodium hyalurate are effective in reducing intestinal adhesion, while chitosan has relative superiority. 3 Chitosan increases the proliferation of EKCs, but sodium hyaluronate exerts no influences on the proliferation of EKCs. Chitosan increases the proliferation of EV304 cells, but hyaluronate sodium showed a reduction in the proliferation of EV304 cells. Both chitosan and sodium hyaluronate can reduce the proliferation of fibroblast cells, but the effect of chitosan is more significant.