Efficts Of Huang Bai Ye On The Primary And Recurrent Genital Infection With Herpes Simplex Virus Type 2 In Guinea Pigs

Object: To investigate the mechanism of HBY on primary and recurrent genital herpes(GH) in Guinea pigs, which proved to be effective to the primary and recurrent GH clinically and inhibit herpes simplex virus type 2 (HSV- II) in vitro in prospective study.Methods: Fifty guinea pigs were divided randomly into five groups, which were all intravaginally infected with HSV- II . After that the animals were administered with HBY(1.41g ml-1, 1.128g ml-1, 0.902g ml-1), and acycllovir or saline for 30 days. When the primary infection lesions disappeared, the perineal skin of metaphane-anaesthetized guinea-pigs was exposed to u.v.-B light for 10 min in order to induce recurrent lesions. All animals were examined periodically for primary and recurrent lesions on the external genital skin, HSV titer in vaginal swab specimens and IFN.Results: HBY reduced primary and recurrent infective lesions on the external genital as the same as acyclovir (P>0.05), but induced more IFN than acyclovir (P<0.01). At the primary infection phase, the virus titers in vaginal swab specimens from the HBY group were higher than those from the acyclovir group, in contrast to the recurrent infection phase. Furthermore, when all of the animals administered with acyclovir or saline appeared recurrent lesions, 5 animals in HBY groups were found no lesions.Conclusions: HBY can directly inhibit the HSV- II from replicating and shedding, and reinforce immunity. As result, HBY has a significant effect on the recurrent HSV- II infection.