The Research For The Expression Of Hepatocyte Growth Factor And C-met In Human Placenta Of Pregnancy Induced Hypertension Syndrome

Preganancy-Induced-Hypertension syndrome(PIH) is a common serious pregnant complication in obstetric department. The incidency rate of it is 9. 4%, it is an important cause of perinatal and maternal mortality. Because its etiology is undefined, it still be one of the most important subjects in Perinatology by now.Abnormal remodeling vasculature is the characteristic pathology in the placenta of PIH, it is also the crucial process of etiology of PIH and result from abnormal function of trophoblast (TB). Hepatocyte growth facor (HGF) is a kind of new cytokine that can regulate various function of TB. It is mainly producted by villous mesenchymal cell,its receptor occurs on the TB and vessel endothelial cells, HGF improved the establishment of remodeling vasculature, placentation and maturity of vessel web by stimulating the proliferation, invasion of cells and morphogen. So, theresearch for HGF is helpful to trace the reason of shallow invasion and clarify the etiology of PIH. The expression of HGF and c-met was measured by Immunohistochemistry here, and contacted with the villous vessel density and umbilicus hemodynamics, in order to elucidate the role of HGF on the etiology of PIH, and then provide theoretical foundation of prevention and treatment.Materials ands Methods 1. All subject were derived into three group: ï¹–he early pregnant group (n=20), woman with normal early pregnancy;?the normal late pregnant group (n=20) ; (3) the PIH group (n=58), according to the diagnostic standard (OBSTETRICS AND GYNECOLOGY, the fifth edit), it is further subdivided into mi Id (n=17), middle (n=20) and severe group (n= 21). All patients were of without other complications of pregnancy and primary hypertension, renal disorders. 2. The biopsies were immediately collected after abortion or delivery, tissue was fixed in 10% formalin and embedded in paraffin wax for subsequent examination. 3. The expression of HGF and c-met were determined using immunohistochemistry and the villous vessel density were determined by the mean count of villous vessels under per high vertex after stained by HE, The parameters of umbilicus hemodynamics were obtained within one week before delivery by ultrasonography.Results 1. There are no significant difference between PIH group and the normal late pregnant group in mean ages , gestational weeks and the basic blood pressure (P>0. 05). The mean systolic and diastolic blood pressure of three group of PIH group were significantly higher than normal late pregnant group (P<0. 05) . The mean weight of newborns in sever PIH group was significantly lower than that of normal late pregnant group (P<0. 05). 2. The stong immunostaining of HGF was observes in villous mesenchymal cells, cytotrophoblasts in the placentas of normal early pregnancy. It also occured in the villous mesenchymal cells and vascular endometricalcells in the placentas of normal late pregnany. The intensity of HGF immunosytaining of mesenchymal cells in the placentas of late pregnant group was lower than that of normal early pregnancy group ( P<0. 05) . 3. The intensity of HGF of villous mesenchymal cells in sever PIH group was significantly lower than that of the normal late pregnant group (P<0. 05) .While it has no qualitative differences between the mild and middle PIH with the normal group ( P>0. 05). There was negative correlation between the intensity of HGF of villous mesenchymal cells and the state of PIH (rs=-0.319 P<0.05) . 4. The stong immunostaining of c-met was observes in trophoblasts in the placentas of normal early pregnancy. It also occured in the trophoblasts and vascular endometrical cells in the placentas of normal late pregnany, no staining was observed in villous mesenchymal cells. The intensity of c-met immunosytaining of trophoblasts in the placentas of normal late pregnant group was lower than that of normal early pregnant group. 5. The intensity of c-met in sever PIH group was significantly higher than that of the normal late pregnant group( P<0. 05), While it has no qualitative differences...