A Experimental Study Of The Mechanisms Of Pinelliae Decoction For Purging Stomach-fire For The Prevention Of Chemotherapy-Induced Gastrointestinal Tract Reaction

Aim: to study the mechanisms of Pinelliae Decoction for Purging Stomach-fire preventing Gastrointestinal(GI) Tract Reaction caused by cisplatin administration in rats, enterochromaffin(EC) cells of the stomach were examined using electron microscopy, and activity of superoxide dismutase(SOD) was examined , activity of monoamine oxidase(MAO) was examined in the duodenum, the concentration of serotonin(5-hydroxytryptamirie, 5-HT) in the brainstem was examined. Intragastric pressure(IGP) was studied.Methods:using pathological technique, electrophysiological technique and biochemical technique to compare EC cells, activity of SOD and MAO, the concentration of 5-HT and IGP in three groups rats(the control group, NS group, TCM group) before or after DDP administration.Results: 1)After DDP administration, as compared with the control group, IGP in NS group was marked higher(P<0. 05), but showed no obvious change in TCM group. 2)After DDP administration, comparing with the control group, activity of SOD and MAO was lower in TCM group and NS group, but the activity of SOD and MAO in TCM group was higher than that in NS group(P<0. 05). Comparing with the controlgroup, the concentration of 5-HT in the brainstem were higher in TCM group and NS group, but lower in TCM group than that in NS group (P<0. 05). 3) After DDP administration, the quantitative and electron-dense of secretory granules of EC cells in TCM group were higher than that in NS group.There was no significant difference between the change of EC cells in TCM group and that in the control group.Conclusion: 1) IGP increased after DDP administration. 2) activity of SOD and MAO in the duodenum decreased after DDP administration.3) concentration of 5-HT in the brainstem increased after DDP administration. 4) quantitative and electron-dense of secretory granules of EC cells in stomach decreased after DDP administration.5)The mechanisms of chemotherapy-induced GI tract reaction may be due to 5-HT release. The mechanisms of Pinelliae Decoction for Purging Stomach-fire preventing GI tract reaction induced by chemotherapy maybe that it can inhibit the release of 5-HT by protecting the intestinal mucosa .