The Effects Of Donor And Recipient VEGF Gene Polymorphisms In Early Stage After Human Beings Kidney Transplantation

Acute rejection is one of major factors, which influence renal allografts survival. Vascular endothelial growth factor (VEGF) is a mitogen for endothelial cells and is expressed widely by renal tissue and T cells. Proliferation of blood capillary endotlielial cells increases the number and the presenting antigen ability of the target cells in the specific immunity reaction. VEGF also influences the expression of adhesion molecules and the migration of cytokings acrossing the endothelium.Objective To study the implication of VEGF: gene polymorphism in predicting acute rejection following kidney transplantation.Materials and Methods 126 recipients of first cadaveric renal transplants engrafted and 63 donors of cadaveric renal transplants at a single centre were selected for the study. Genomic DNA were purified from the recipients' blood and donors" lymphocyte suspension by absothing medoths. The homozygote. muta-tional heterozygote and mutational homozygote at nucleotide position -1154 were detected by Dye-Terminate-Cycle-Sequencing medoths. The distribution frequencies of different VEGF genotypes and the influenced of VEGF geng polymorphism on renal transplantation were analyzed. All of these individuals donated two kidneys to local recipients. When one recipients had acute rejection and another had no rejection, the donor had one instance acute rejection and one no rejection instance. When the both of recipients had acute rejection, the donor had two acute rejections instances. On the contrary, the calculation way is the same.Results In healthy groups and patient groups, the frequencies of VEGF -1154 high producing genotype was 57.1% and 60.3% respectively, but nodifference was found between two groups(P>0.05). Acute rejection occurred in 32.5%; median time to first rejection episode was 18.5d at 6 mo. The frequencies of recipients' VEGF -1154 haplotype G and phenotype G in acute rejection groups was 85.4%, 95.1%. which was significant higher than the control groups'(P<0.01 , P<0.05 respectively). The frequencies of donors' VEGF -1154 haplotype G and phenotype G in acute rejection groups was 82.9%, 95.1% . which was also significant higher than the control groups' (P<0. 01 , P<0.01 respectively). In HLA-DR-mismatched tansplantation groups, the frequencies of donors' and recipients" VEGF -1 154 G/G high producing genotype w:as 66.7%, 79.2%, which was significant higher than the control groups'(P<0.01 , P<0.01 respectively). However, in HLA-DRCK Imismatched tansplantation groups, no difference was found between acute rejections groups and control groups in the donors' VEGF -1 1 54 genotypes. The same result was observed among the recipients groups.Conclusion When HLA-DR-mismatched It may beneficial to predict the risk of acute rejection to detect the donors' or recipients' VEGF genotype at -1154 position before renal transplantation.