Experimental Study Of Chitosan In Preventinc Postoperative Abdominal Adhesion

Objective: 1. To investigate the process of postoperative abdominal adhesion generating in rats. 2. To detemine the effctiveness of chitosan in preventing intrapertoeal adhesion and it's mechanism.Methods: ninty-six rats was utilized to set up animal models of intra-abdomind adlesion. After scratching cecal peritoneum and the abdominal wall apposition on rats, We radomly divided the rats into two groups. And the peritoneum were covered with chitosan 0.05ml or saline solution. Before the skin closured a soft drainage-tube, 4mm in diameter, was put. After 6h the exudate was extracted from drainage-tube, with the varying posture. A small amount of normal saline was injected via the tube to wash the abdominal cavity. The collected exudate was disposed and counted the number of WBC. The drainage tube was taken out. After 3 days, 6days, lOdays and 14days the rats were killed for examining the intra-abdominal adhesion. Then the abdominal wall and opposed cecum were: dissected, the expression of TGF-B1 were examined utilizing immunohistochemsthy The formation of scar were obseved with light microscope.Results: 1.In the Normal saline group the amount of exudate=(1.25±0.09)ml the number of WBC(x103)=(23.1±6.6)mm3, In chitsoan group however the amount of exudate was 0.52±0.04ml (P<0.01),the number of WBC(xl03)=(10.6±4.2)mm3(P<0.01). It indicatedthat chitsan could decrease exudate and inhibit inflammation. 2. The key period of intra-abdomind adhesion is from 6th day to the 10th day. After using chitosan. The hurted peritoneum's adhesion was significantly reduced (P<0.01). The scar tissue reduced and the expression of TGF-B1 were reduced (P<0.01).Conclusion: Chitoson appears to reduced the extent of postoperative intraperitoneal adlesion. It's mechanism may relate to the inhibiting to the expression of TGF-B1 Then inhibit to the growth of fibroblasts.