The Effects Of TGF-β1 And BFGF On Fibronectin Expression Of Human Peritoneal Mesothelial Cells

ObjectiveNormal human peritoneum was covered by the peritoneal mesothelial cells and the extracellular matrix. The peritoneal mesothelial cells can prevent tumor cells in the peritoneal adhesion .Free intraperitoneal secretion of gastric cancer cells through a variety of cytokines damage caused peritoneal mesothelial cells fibrosis. Peritoneal fibrosis has provided a suitable environment for peritoneal metastasis of gastric carcinoma To further explore gastric cancer cells to the impact of the peritoneal mesothelial cells, we explore the effects of TGF-β1 and bFGF on fibronectin expression of human peritoneal mesothelial cellsMethodThe experiment is divided into experiment set and matched control .The peritoneal mesothelial cell was stimulated by 1 ng / ml ; 10 ng / ml ; 100 ng / ml TGF-β1 and 1 ng / ml ; 10 ng / ml ; 100 ng / ml bFGF. We adopt MTT dyeing method to observe the peritoneal mesothelial cell growth curve .We observe the fibronectin expression in the pure liquid of the peritoneal mesothelial cell by ELISA method .We used RT-PCR method to observe the fibronectin mRNA expression in the peritoneal mesothelial cell.ResultThe peritoneal mesothelial cell expressed fibronectin. Compared with control, the peritoneal mesothelial cell's proliferation rate was significant increased after stimulated by TGF-β1 and bFGF (P<0.05 ). The increase were in direct proportion to the concentration of TGF-β1 and bFGF. But there is no difference between the same concentration of TGF-β1 and bFGF. The Fibronectin expression of the peritoneal mesothelial cell was obviously increaseed stimulated by TGF-β1 and bFGF (P<0.05 ) and the increase was in direct proportion to the concentration of TGF-β1 and bFGF. But there is no difference between the same concentration of TGF-β1 and bFGF. Fibronectin mRNA expression was higher at afferstimulation by TGF-β1 and bFGF compared with control group.ConclusionTGF-β1 and bFGF can stimulate the peritoneal mesothelial cell's proliferation. They can enhance fibronectin expression of the human peritoneal mesothelial cell and raise the expression of Fn mRNA of the human peritoneal mesothelial cell. TGF-β1 and bFGF were advantageous to the Peritoneal fibrosis and Provide feat environment for the metastasis of gastric neoplasm.