The Proliferation Of Hematopoietic Progenitor Cells In Normal Human, Mice And Aplastic Anemia Mice Enhanced By Panaxadiol Saponins

Objective To investigate the proliferation effects of Panaxadiol saponins (PDS) extracted from ginseng herb on hematopoietic progenitor cells in normal human, mice and aplastic anemia mice.Methods The colony formation of CFU-GM, CFU-E and CFU-MK in both normal human and mice were observed by semi-solid culture of bone marrow in the density of PDS 5, 20, 50 and 100mg/L. Balb/c mice with immune-mediated aplastic anemia were treated with PDS at higher, middle and low dosages (2.0, 1.0 and 0.5mg per mouse per day) for 10 days, the peripheral white blood cells (WBC) and platelets (PLT) were counted, the femora were fixed and cut for pathological examination, and bone marrow of CFU-GM, CFU-E and CFU-MK were incubated.Results (1) The proliferation of haemopoietic progenitor cells from normal human could be promoted by PDS. The colony numbers of CFU-GM, CFU-E and CFU-MK were increased by 30.1 2.8-47.7 2.0%, 22.4 1.6 ~ 35.0 1.2% and 22.9 1.6 ~ 35.1 2.5% respectively over those of non-PDS control(all of P <0.01). (2) PDS also could enhance proliferation of haemopoietic progenitor cells from normal mice. The colony forming efficiency of CFU-GM, CFU-E and CFU-MK induced by PDS were 27.2 1.9 ~ 45.0 1. 8% 鈥?34.1 1.4% ~ 39.7 l.l%and 16.9 1.3 ~ 23.9 1.4% respectively more than those of non-PDS control(all of P <0.01). (3) The structure of hematopoietic net was destroyed and replaced by fatty tissue in model mice with aplastic anemia. While, the net tissue of marrow was finer and filled by hematopoietic cells after being treated by PDS. It meant the damaged hematopoiesis function of bone marrow could be improved by PDS. The counts of peripheral WBC and PLT in model mice were distinctly decreased being compared with normal mice(both P<0.001), while they were significantly increased in three groups of PDS-treatment (all of P <0.01). (4) The colony numbers of CFU-GM, CFU-E and CFU-MK in model mice were distinctly decreased(P<0.01), while after treatment with PDS they were significantly more than those of un-treated model mice(all of P <0.01).Conclusion PDS was an effective component for enhancing hematopoiesis within total saponins of panaxginseng. PDS could promote proliferation threelineages of myeloid, erythrocytic and megakaryocytic progenitor cells. It was useful not only for normal progenitor cells, but also for those in haematological diseases such as aplastic anemia. These results provided an evidence for application of PDS as an effective hematopoietic material in the future.