Research Of Radiofrequency Abalation Pre-management For Liver Resection Margin On Rabbit Model

Background and objectiveHepatolobectomy procedure remains a high risky surgical procedure nowadays. Especially, intraoperative bleeding is the main problem yet. It is related directly to high post operation complications and low long-term survival rates. So reducing and controlling the bleeding is the prime task during hepatolobectomy procedure. Radiofrequency technologies have been used for much a few years and has been improved greatly for recent years, they has now been regarded as mini-invasive reliable and safe methods to use. But these methods are confined to treating primary and metastesis liver tumor. It' s been rarely reported about the application of using radiofrequency on surgical margins of liver.Material and Methods28 healthy New Zealand big rabbits provided by animal center of Zhejiang University . The body weight were ranged from 2. 0 to 3. 0 kilogram , no restriction to sex , and was randomly divided to 4 groups : A group :liver cutting ; B group : radio-frequency ablation and liver cutting ; C group : portal block and liver cutting ; D group : radio-frequency ablation and portal block and liver cutting . Anesthesia was used with Thiopental Sodium lOOmg/kg, im . The time of radiofrequency ablation was 4 minutes , output power was 50~60w , the site was in the centre of anterior lobe of left liver . portal block used pringle' s method , 15 minutes . we used peng' s multifunction operation dissector (PMOD) cut the liver and cut the half of the anterior lobe of the left liver . recorded the operation time and bleed volume during operating and Estimated the ALT and AST pre and postoperation .and observated the pathologic change of liver . the data analyzed by T-test .ResultGroup B could reduce operative bleeding more efficiently than Group A, there are no apparent difference in intraoperative bleeding volume between Group D and Group C, also between Group B and Group C. The operative time decreased more significantly in Group B , C, D than Group A, and there are no difference in operative time within these three groups.Serum ALT and AST change:ALT elevated significantly in each group postoperatively after 1-48 hours or 1-24 hours, and it reach the peak in 12-24 hours postoperatively, and go back to preoperative level in 3-7days postoperatively.. there are no significant difference in ALT and AST change between Group B and Group C, there is more obviously difference about elevated ALT and AST in Group D than in other Group.ConclusionRadiofrequency abalation pre-management on liver resection margins could reduced efficiently operative bleeding during liver resection operations. It is a new assistant technology applying intraoperatively for liver surgical procedure.