Studies On The Constituents Of Pseudostreblus Indiea Bur

Bark Of Pseudostreblus indica Bur. has been used as a folk remedy of hemorrhage and hematemesis duo to visceral injury in yunnan province. There are only two investigations in chemical in china. In order to find new compounds and optimization of the resourse , we have an investigation on it.The Pseudostreblus indica Bur. was collected and identified by Tao guo da who is a researcher in yunnan province. The dried and mealy materials were sepal with 95% ETOH and degreased with petroleum ether, then the sample was extracted by Diethyl ether and Ethyl acetate by turns. The Ethyl acetate's friction was chromatographedon the column of silica gel with the mixed solvent of Petroleum ether as gradient elution, we got six compounds from it and identified the structure of four compounds by(IR,MS, UV,1H-NMR,13C-NMR) spectrum and chemical evidences. There are bergarpten I , umberlliferone II , tanshione II A III and the main chemical constitument of a compound.In the way of pharmaceutical technology researching , the exation process for Pseudostreblus indica Bur. was studied. The optimum process was chosen by L9 (34) orthogonal text. The optimum process was as follow : 10 times amount of 60% ethanol , refluxing and extracting for three times , one hour every times for Pseudostreblus indica Bur. In thisstudy, to observe the protective effects of extract of the diethyl ether on injuried primary cultured rat hepatocytes induced by CCl4. extract of the diethyl ether significantly improved cell viability, inhibited the decrease of SOD activity and the increase of ALT, LDH, MDA, NO levels. The result is that extract of the diethyl ether protected effectively the primary cultured rat hepatocytes against CCl4-induced injury.