The Fingerprint Research Of Medical Material Of Phyllanthus Urinaria L.

The fingerprint reserch of medical material of Phyllanthus urinaria L.The modern analytical technique is applied to the reserch of fingerprint by using figures to describe the internal chemical information of the traditional Chinese medicine. The fingerprint can reflect the different of description and quantity of chemical information. It can be used to control the quality of the traditional Chinese medicine and drugs preparati on through the full-scal e and integrative information. It' s a better method to control the quality of traditional Chinese medicine which could be ratified in the international.In Chinese civil, Phyllanthus urinaria L. is used to treat the coprorrhea, alv laxat, urinary tract infection, oedema nephriticum, icterohepatitis, and so on. In the past few years , it is mainly used to cure the Type B viral hepatitis and known as a potential, safe and effective plant amedica. In this articles, we used two of the internal phenolic acid ingredient-cotilagin and gallic acid-as control article. We used different extract methods and considerated two mobile phase systems-MeOH-H2O and acetonitrile-H2O.For the purpose of optimize the mobile phase systems, we added micromolecule organic acid, such as acetic acid, trichloracetic acid and tetrahydrofuran to improve the peak shape and resolution. We ascertained the the mobile phase systems and elution grad. The water-solubility and liposolubi1ity ingredient were maintained proper resolution under the circumstance. All chromatographic peak area score are stable. Through the process of evelopment analysis, comparision evaluation,andverification,we established the HPLC fingerprint of Phyllanthus urinaria L. and compared Phyllanthus urinaria L. which yielded from diferent places. The consequence indicate that the fingerprint can reflect the resemblance of the medical material and provide re I iable evidence to control the quality of Phyllanthus urinaria L. At the same time ,we compared the distinction of fingerprint of Phyl lanthus urinaria L. which yielded from different places and found thatthere are no significant difference. These minor difference may he caused by the different living surrounding, collected season, the period of provision, and the homogeneity of taking of samples and the condition of extraction which we can't hold completely at equal pace.