The Effect Of Phyllanthus Urinaria Extract On Schistosomiasis Induced Hepatic Fibrosis And Intervention Mechanism On IL-13/miR-21/smad7 Signaling Pathway

Part ? The effect of Phyllanthus Urinaria(PU)preparationHaizhuyigan capsule on schistosomiasis liver fibrosis patients with serum IL-13Objective This part is to investigate the PU preparationHaizhuyigan capsule on schistosomiasis liver fibrosis patients with serum IL-13.Methods 20 schistosomiasis liver fibrosis patients were randomly divided into treatment group and control group.10 cases in the control group patients were gave the foundation treatment and the treatment group patients were not only gave the basis foundation treatment but also added Haizhuyigan capsule.The course of the treatment in the two groups is one month and the Elisa method was used to detect the levels of serum IL-13.Results After one month treatment,the treatment group and control group patients serum IL-13 levels were significantly lower than before treatment(P<0.01).And after treatment,the serum levels of IL-13 in the treatment group was lower than control group(P<0.05).Conclusion The PU preparation-Haizhuyigan capsule could decrease the serum IL-13 level on schistosomiasis liver fibrosis patients,which provides theoretical basis for schistosomiasis liver fibrosis.Part ? Study of PU extract on the hepatic fibrosis mouse model infected with Schistosoma japonicum in liver histopathologyObjective The study was to investigate the anti-fibrotic effect ofPU extract on a schistosomiasis-induced hepatic fibrosis mouse model in liver histopathology.Methods 50 BALB/c mice were randomly divided into PU extract high concentration group,middle concentration group,low concentration group,model group and normal group.The first four groups were infected by Schistosoma japonicum cercaria.After 4 weeks infection,the groups were given appropriate medication,control group received saline.Meanwhile,each group was intragastric administration one time every day for4 weeks.Then we observed living conditions,and recorded the mice body weight.Specimens were detected by immunohistochemistry the expression of CTGF(connective tissue growth factor),HE(Hematoxylin-eosin)staining,MASSON staining pathological changes in the liver of mice.Results The degree of fibrosis,the expression of CTGF protein in the three PU extract groups was less than model group levels,the differences were statistically significant(P < 0.05).Conclusion PUextract can significantly reduce the liver pathology injury induced by Schistosoma japonicum cercaria,relieve the degree of liver fibrosis.Part ? Effect of PU extract on the IL-13/mi R-21/smad7 signaling pathway in a schistosomiasis-induced hepatic fibrosis mouse modelObjective The study was to investigate the anti-fibrotic effect of PU extract in a schistosomiasis-induced hepatic fibrosis mouse model by IL-13/mi R-21/smad7 Signaling pathway.Methods 50 BALB/c mice were randomly divided into PU extract high concentration group,middle concentration group,low concentration group,model groupand normal group.The first four groups were infected by Schistosoma japonicum cercaria.After four weeks infection,the groups were given appropriate medication,control group received saline.Meanwhile,each group was intragastric administration one time every day for 4 weeks.The living conditions were observed.The IL-13 level in serum was examed by Elisa assay.Real-time PCR was employed to detect the m RNA levels of mi R-21,Smad7 and CTGF,and western blotting was used to examine the protein levels of Smad7,Smad1,p-Smad1,Smad2,p-Smad2,ERK1/2,p-ERK1/2 and TGF-?receptor I.Results Coppared with normal group,the m RNA expression of mi R-21 and CTGF increased notably(P < 0.01),the m RNA and protein expressionof Smad7 decreased(p-ERK1/2 and TGF-? receptor I)and the protein expression of p-Smad1,p-Smad2,p-ERK1/2 and TGF-?receptor I also increased(P < 0.01).Compared with the model group,increasing concentrations of PU extract inhibited the m RNA expression of mi R-21,promoted smad7 protein expression(P < 0.05 or 0.01).Moreover,PU extract significantly reduced the protein levels of p-smad1,p-smad2,p-ERK1/2,CTGF and TGF-? receptor I(P < 0.05 or 0.01).Conclusion PU can significantly inhibit Schistosome liver fibrosis through IL-13/mi R-21/smad7 pathway.