| Labeled immunoassay are the total conception of a series of highly sensitive and specific examine techniques, and have been widely applied in the basic medicine and clinic field for their special advantages. Time-resolved Fluorescence Immunoassay (TRFIA) has become one of the hotspots in the field of labeled immunoassay application. Hepatitis B is a damage of hepar by the Hepatitis B virus .Now it is a epidemic disease in our country .The following results have been obtained from experiments:l.To establish a two-site time-resolved fluoroimmunoassay(TRPIA) of hepatitis B virus surface antigen(HBsAg) hepatitis B virus surface antibody(HBsAb) and hepatitis B virus e antigen(HBeAg) based on the direct sandwich technique.The assay ranges of standard curves were 0-300ng/ml 0-1280mIU/mland 0-16NCU/ml,respectively, the assay sensitivities were 0.05ng/ml 0.44mIU/mland 0.03NCU/ml The within-run coefficient variations for standard samples were less than 10%. Compared this method with radioimmunoassay,they were interrelathed with each other Repression coefficient was 95%.2.To establish a neutraJizing-inhabition time-resolved fluoroimmunoassay(TRFIA) of hepatitis B virus e antibody(HBeAb) and hepatitis B virus core antibody(HBcAb) based on the competion .The assay ranges of standard cures were 0-16NCU/mland 0-8NCU/ml.The within-run coefficient variations for standard samples were less than 10%. Compared this method with radioimmunoassay,the sensitivities were 92.8% and 93.3%.In conclusion, the diagnostic methods we developed for immunological markers of hepatitis B virus by using time-resolved fluoroimmunoassay, Eu-DTTA as label, have wide assay ranges, high sensitivity and specificity. They would have widely application in the further research work and clinical diagnosis.
|
PDF Full Text Request