Expression Of Cancer Testis Antigen SCP-1 In Hepatocellular Carcinoma

Objective: To detect the expression of Cancer-Testis Antigen SCP-1 in Hepatocellular carcinoma (HCC), and to discuss the possibility of this antigen as a vaccine for immunotherapy.Method: With Reverse-Transcription Polymerase Chain Reaction (RT-PCR) the expression of SCP-1 in cancerous and corresponding paracancerous tissues from Forty-five patients with HCC was tested, and statistic analysis was performed. The SCP-1 positive specimens were immunohistochemically detected by SP method with SCP-1 specific monoclonal antibody.Results: The expressive frequency of SCP-1 in HCC is 13.3% (6/45). There is no expression of SCP-1 in any corresponding paracancerous tissues. SCP-1 proteins were detected in the six SCP-1 mRNA positive specimens, which are highly heterogeneouse. Statistic analysis showed that the expression of SCP-1 was correlation with tumor size and livercirrhosis (P<0.05) , but there was no correlation with pathological stage, serum AFP level and infection of hepatitis B virus (P<0.05).Conclusion: The expression of SCP-1 in HCC is relatively low, which implied that SCP-1 should combine with other tomor antigens as polyvalent vaccine to get good effect in HCC immunotherapy.