Inhibition Function Of Vascular Endothelial Growth Factor Antibody On The Growth And Angiogenesis Of Human Osteosarcoma

Objection: To observe the inhibition of vascular endothelial growth factor (VEGF) antibody on the growth and angiogenesis of human osteosarcoma. Methods: We subcultured the human osteosarcoma cell line U-2 OS, and the cell in logarithmic phase of growth were inoculated in nude mice by subcutaneous injection of 107 cells. The tumor was cut when it grew by about 1cm, and the tumor tissue was cut into about lmm pieces. We transplanted the tumor tissue pieces into 16 nude mice subcutaneous to establish human osteosarcoma model for studying anti-angiogenetic therapy. Nude mice were randomly divided into two groups after the tumors grew up, the treated group receiving 100ug anti-VEGF antibody for each mouse which was locally injected in around the tumor and in the tumor once two days, control group receiving PBS. The length, width and weight of tumor were measured. Microvessel density(MVD) by means of CD34 immunohistochemistry was detected. Results: There were significant volume and weight differences of tumor between treated group and control group of nude mice, the inhibition rate of treated group reached 54.05%, and there was a significantty lower MVD in treated group than in control one. Small tumor necrotic areasdiffuse in HE stains of treated group, control group seldom has necrotic areas. Conclusions: The tumor MVD decreased and the growth of osteosarcoma was inhibited after the application of VEGF antibody, blocking of VEGF-VEGFR signal conduction way can inhibit osteosarcoma angiogenesis.