The Experimental Research Of RuJianHuGanPian On Hepatic Fibrosis

Objective: To probe the effect of RuJianHuGanPian on experimental hepatic flbrosis rats.Method: Establish the rat model of hepatic flbrosis by using 40 percent carbon tetrachloride and alcohol. First part (prophylactic experiment): 38 female Wister rats were randomly divided into three groups : normal group(n=8) , non-preventive group(n=15) , preventive group(n=15). Forty percent carbon tetrachloride (CC14) dissolved in peanut oil was injected subcutaneously to rats in non-preventive group and preventive group ( First 5 ml/kg , then 3ml/kg ,once threedays) for 6 weeks to produce hepatic flbrosis model . Normal group received Same dose Saline as a control .At the same time, preventive group rats were ingested with solution of RuJianHuGanPian (1.55g/kg daily ,5 times a week ),while those of normal group and non-preventive group only received same dose saline . 20% alcohol was administered to the non-preventive group and preventive group for a long time. After 6 weeks, all rats were killed. Serum hyaluronic acid, laminin , IVcollagen level and semi-quantitative histopathological area , percentage of collagen fibril and the stage of fibrosis were compared in each group .Second part (therapic experiment): 58 rats were divided into: positive control group, RuJianHuGanPian therapic group, Colchicine group. All rats were deduced into hepatic fibrosis model by using same method as at first part. After model had been established, saline, RuJianHuGanPian, Colchicine(1.5mg/kg 5 times a week )was administered respectively .At the end of the experiment, same indices were analysed . -Result: Experimental research: (1) First part (prophylacticexperiment ): Compared with non-preventive group , Serum hyaluronic acid, laminin , IVcollagen level and stage of fibrosis were lower in normal group and preventive group which total area , percentage of collagen fibril were decreased .(2)Second part (therapic experiment): The level of all parameters was higher in positive control group than that of RuJianHuGanPian therapic group and Colchicine group. There was not significant difference between RuJianHuGanPian therapic group and Colchicine group.Conclusion: 1. RuJianHuGanPian is effective in protecting against experimental CC14 induced liver fibrosis rats.2. RuJianHuGanPian can decrease the level of serum hyaluronic acid, laminin , IVcollagen and semi-quantitative histopathological area, percentage of collagen fibril in experimental CC14 induced liver fibrosis rats.