| Basically speaking, the dysfunction of cell cycle control cause irregular proliferation and differentiation that lead to malignant changes.Cycling Dl protein was associated with cell cycle regulation acting on Gl phase, acting with many other proteins promoting cell into S phase. Cyclin Dl protein was believed to be a prooncogene, its over - expression occurred in man-y kinds of tumors.p27 was a tumor suppressor gene, countering act with cyclin Dl. As a negative cell cycle regulator, p27 inhibit tumor cell proliferation by hinder it from Gl to S phase.The expression of cyclin Dl and p27 in many kinds of tumors was associated with their biological behaviors, that is cyclin Dl over expression and p27 deletion or less expression in tumors. It has not been reported their expression and role in vulvar squamous cell carcinoma.Vulvar squamous cell carcinoma accounts for 3 -5% of all female genital tract cancers, and 1 -2% of all woman cancers. The rate of vulvar intraepithe-lial neoplasia ( VIN) is increasing in recent years, Non ?neoplastic epithelial disorders ( NNED) was traditionally regarded as a pro - cancerous disease, which now people find it has a tumorigenesis trend by epidemic research, but it is not confirmed by molecular pathologic method.We tested the expression of cyclin Dl and p27 in vulvar squamous cell carcinoma, VIN, NNED and normal vulvar skin, in order to find their relations.Material and Methods1. Clinical material; Cases from the First Hospital of China Medical University were collected between January 1994 and June 2003, including 20 cases of vulvar squamous carcinoma; 40 cases of NNED, 20 of them were squamous cell hyperplasia, the other 20 were lichen sclerosus; 30 cases of VIN, 10 VIN I ,10 VIN II ,10 VIN III; 5 cases of normal vulvar skin.2. Immunohistochemistry was employed in this study to detect expression of cyclin Dl protein (mouse anti -human cyclin Dl monoclonal antibody; ready to use, Maxiam Biotech) and p27 protein ( goat anti - mouse IgG, ready to use, Maxiam Biotech). All procedures were implemented according to the product illustrations. We used PBS instead of primary antibody as negative control.The immunostaining of p27 and cyclin Dl was localized to the nucleus and cytoplasm. Statistical evaluation was performed using chi - square to differentiate the rates of different groups. SAS version 6.11 software was employed to analyze all data.Results1. The cell differentiation degree was not correlatd with age, 60% (12/20) of vulvar squamous carcinoma were high differentiation no matter in any age groups.2. Positive rate of p27 and cyclin Dl among different groupsp27 protein positive rate was 100% (5/5) in normal vulvar skin, 87. 5% (35/40) in NNED, 83.3% (25/30) in VIN and 40. 0% (8/20) in vulvar squamous carcinoma. The positive rate of p27 in normal vulvar skin was higher than that in VIN and vulvar squamous carcinoma, but the difference was not significant; There were significant differences among vulvar squamous carcinoma, normal skin, NNED, and VIN ( p < 0.01).The positive rate of cyclin Dl was 0% (0/5) in normal vulvar skin, 35. 0% (14/40) in NNED, 43.3% (13/30) in VIN and65.0% (13/20) in vul-var squamous carcinoma. There were significant differences of cyclin Dl expression among normal wulvar skin with vulvar squamous carcinoma, NNED, and VIN (p <0. 05) ; There were also significant differences among vulvar carcinoma with NNED and VIN ( p < 0.5 ) ; But there was no significant difference between NNED and VIN ( p > 0.05).3. Positive cell proportion of p27 and cyclin Dl among different groups p27 protein positive cell proportion was 97.4% in normal vulvar skin, 73.6% in NNED, 66. 27% in VIN and 11. 9% in vulvar squamous carcinoma. There were significant differences among vulvar squamous carcinoma with nonnal vulvar skin, NNED, VIN ( p < 0. 01 ) ; the differences among normal vulvar skin, NNED, and VIN were not significant.Cyclin Dl protein positive cell proportion was 0% in nonnal vulvar skin, 23.65% in NNED, 17.23% in V...
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