| Morbidity and mortality of the lung cancer increased very highly . It has been one of the meligant tumor that threaten humans health and life. All the world. About 900,000 man and women died of lung cancer every year. It has been the first tumor in all meligant tumor to human death, there are many diff-culty for us to diagnose lung cancer. Telomerase activity is the most general marker for the indentification of human cancer, the analiysis of telomerase mR-NA expression in serum and blood mononuclear cells is attractive as a potential tumor marker. Although a good correlation between telomerase reverse tran-scriptase (hTERT) expression and telomerase activity has been reported. In this study, we use RT-PCR detected peripheral monouclera hTERT of 31 lung cancer and 17 benign lung diseases and 11 normal persons. In order to find a new way to diognose the lung cancer.Materials and methods1. Cease selecting: all the cases were selected from the patients that treated and diagnosed in the second hospital of china medical univinity , it in-cludes30 lung cancer: and 18 benign lung disease , the contrast were 11 normal persons, the age of those case were:lung cancer: 35-78(60.4 + 14. 53 ) ,benign lung disease: 14-36(58.1 +11.3) ,normal presons:22-72(50 + 18.8).2,Blood monouclear cells were obtained from 31 lung cancer ,18 benign lung disease and 11 normal persons. In a 10ml tubes contains heparin and a density gradient medium. RNA was isolated from the blood monuclear cells use total RNA Extraction system II, hTERT primer was design with primer 5.0 soft-ware, hTERT-fw:5'-TAT GCC GTG GTC AAG G-3', hTERT -Rv :5'-GCG TGD GTG AGG TGA GGT CT-3',the longth was 328bp , B-actin pimer: Fw:5' GAT TGC CTC AGG ACA TTT CTG-3'; Rv:5'-GCG TGG GTG AGG TGA GGT CT -3'. the longth is 690bp. cDNA synthesis using RT-PCR kit , performed for 1 minutes at 65C 5 minutes at 30C 15to30minutes at 30C ,to65 C 30 minutes at 65 5 minutes at 98 C 5 minutes at 5C. Those cDNA1ul add to dd. H2O, 10buffer 1. 25ul, dNTP1ul, hTERT-fw 0.1ul, hTERT -Rv0.1 ul, PCR programming was 3 minutes at 93 C for enzyme activation , followed by 35 three-step cycles (45 second at 94 C ; 1 minutes at 57. 6C ; 1 minutes at3 PCR product was meausured by electrophoresis in 2% sepharose and to observed and take photo with violet gl scanner, use the system 1 D Kadak imaging anilysis and cumpute hTERTmRNA and B-actin gene peak volume, the ratio was used to statistical treatment. Statistical treatment : the SPSS 10. 0 software was used to statistical treatment for one way analysis of variance and test of homogeneity of variances .ResultThe peripheral blood monouclear hTERT in lung cancer and benign were; 0. 9193 +0.1239; 0.5446 +0.1459; 0.4517 +0.0318. And significant difference was found (p<0.001); in benign and health no significant difference was found(P >0. 05). the sensitity of hTERT was: 83. 87% and specificity was: 100% and positive predictive value was 100% , negative predictive value was 78.26%; Accuracy:88.8%.ConclusionDetecting hTERTmRNA of peripheral blood mononuclear cells is one of important methods for lung cancer diagnosis. hTERTmRNA quantitation in blood mononuclear cells could be viewed as a promising tumor marker, In this study results show that measurement of hTERTmRNA in PBMCs discriminates betweenhealthy subjects and lung cancer and support the idea that a diagnostic or prognostic test for cancer might be developed based on this genetic marker in blood.
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