| OBJECTIVE To explore the effect of Liqustrazin on the no-reflowphenomenon in rabbits undergoing acute coronary artery occlusion-reperfusion.METHODS Rabbit' s acute ischemia- reperfusion model was carried out byligation of left post coronary descending artery and removing the ligation after occlusion of blood flow for 90min, followed by 120 min of reperfusion. Ligustrazine (group I , 2mg/kg bolus iv+0. 3mg.kg-1.min-1,n=15), Nicorandil (group II, 0.2.kg-1.min-1, n=15) and 0. 9% Nad saltsolution (NS) (group III, 0. 3ml.kg-1.min-1, n=15) were given intravenously after restoration of coronary blood flow and maintained for 120 min, respectively. The changes of electrocardiogram (ECG), activity of CK and LDH, amount of MDA and SOD in plasma were observed following acute ischemia and restoration of flow. 120 minutes after reperfusion, each rabbit was killed and its left ventricle (LV) was cut into 8-10 slices parallel to the atrioventricular groove. The no-reflow (NR) area and the infarct size (IS) were determined by thioflavin S and TTC staining at the end of the experiment respectively. The extent of NR and IS were expressed as percentage of LV.RESULTS Ligustrazine and Nicorandil both resulted in a significant reduction in IS, NR, plasma levels of CK, LDH and MDA, while increase plasma level of SOD, in comparison with control animals.The extent of elevated ST segment in ECG (expressed as ESTI) in group I and II were both lower than that of group III (group I vs groupIII:P<0.01;group II vs groupIII: P<0.01) at the end of experiment. Comparing with nicorandil, Ligustrazine result in asignificant decrease in CK(p<0.05) and LDH (p<0.01), while there is no significant different in the effects on MDA, SOD and STI between two groups (p>0. 05).CONCLUSION Ligustrazine can significantly decrease NR area and infarct size, make an important contribution to myocardial injury during postischemic reperfusion, and its effects are similar to that of nicorandil.
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