Study On Serum Levels Of Timp-1 In Patients With Diabetic Nephropathy

Objective To investigate the serum levels of tissue inhibitor of meta-lloproteinase-1 (TIMP-1) in different clinical stages of patients with diabetic nephropathy,to explore the role of TIMP-1 in the development and progression of diabetic nephropathy.Methods Eighty people were sampled to conduct this study, including normal control group (NC,n=20),type II diabetic mellitus(DM,n=20),clinical stage of diabetic nephropathy(DN,n=20)and renal failure stage of diabetic nephropathy (DNF, n=20) .Serum concentrations of TIMP-1 from all subjects were detected by enzyme-linked immunosorbent-assay (ELISA)then analyse statistically the difference of TIMP-1 levels between groups and between sex with analysis of variance, q-test (Student-Newman-Keuls) and t-test.The relativity of TIMP-1 level and age were checked with correlation analysis and t-test.Result Serum levels of TIMP-1 were 825.97 137.34ng/ ml, 1259.05 218.47 ng/ml,1508.31 282.57ng/ml and 213 1.42 441.25ng/ml in NC, DM, DN and DNF respectively. Levels of TIMP-1 in patients with DM, DN and DNF were more significantly elevated than those in normal control group ( P<0.01 ) . Patients with DN had higher TIMP-1 level compared with DM patients (P<0.05). In addition,patients with DNF had significantly higher TIMP-1 level compared with DN patients(P<0.01).There was no statistical significance between sex (P<0.05) , but the concentrations of TIMP-1 were associated with ages in DM and DNF groups ,the coefficient of correlation was 0,4866(P<0.05)and 0.6229(P <0.01), respectively.Therewas no apparent statistical difference between the coefficient of correlations.Conclusion Serum levels of TIMP-1 were gradually increasing with the progression of diabetic nephropathy,The results strongly suggested that TIMP-1 may contribute to vascular remodeling during the development and progression of high blood pressure and atherosclerosis. Meanwhile, TIMP-1 may mediate remodeling of glomerular basemment membrane(GB M) and glomerulos-clerosi and plays an important role in the development and progression of diabetic nephropathy.