Application Of Immuno-PCR For Anti-P53 Antibodies In Diagnosing Breast Carcinoma

In order to establish a sensitive immuno-PCR assay for detecting serai anti-P53antibodies, investigate the relation of anti-P53 antibodies in patients with breast carcinoma to P53 protein expressions in tissue and clinical significances and afford a practical tool in diagnosis of breast carcinoma clinically. SaHIgG was coupled to PNCa DNA by chemical cross-linker PEI and SaHIgG-DNA probe was constructed. The SaHIgG-DNA probe constructed by chemical cross linking was identified and the results indicated its antibody and DNA activity were up to grade in quality and adapt to immuno-PCR completely. The SaHIgG-DNA probe is anti-antibody (Ab2) and therefore has wide applications in immuno-PCR.The positive rate of anti-P53 antibodies by immuno-PCR was 39.5 % in 86 sera of patients with breast carcinoma, none of anti-P53 antibody was found in 40 non-carcinoma and 120 health controls, The statistics results indicated the positive rate of anti-P53 antibodies was significantly higher in patients with breast carcinoma by immuno-PCR than non-carcinoma and health controls (P<0.01) and there was no difference bwtween patients with non-carcinoma and health controls (P>0.05). The specificity of immuno-PCR for anti-P53 antibody was 100%, therefore anti-P53 antibodies can be used as a seriological tumor marker.P53 protein expressions were detected by immunohistochemistry (IHC) assay. The positive rate of anti-P53 antibodies was 64.2% in 53 cases of sera of breast carcinoma with positive P53 protein expressions and was significantly higher than that of negative P53 protein expressions, p<0.01. The results indicated anti-P53 antibodies in sera was positively correlated to overexpressions of P53 protein in tissue.The positive rate of anti-P53 antibodies by ELISA was 18.6%, The detecting rate of anti-P53 antibodies by immuno-PCR (39.5%) was significantly higher than that of ELISA (PO.01).Respectively. Immuno-PCR for anti-P53 antibodies in sera assay was simpler than immunohistochemistry assay for P53 protein expressions in tissue and therefore was a practical tool.