Study On ELISA For Detecting Anti-desmoglein1Antibody And Anti-desmoglein3Antibodyin Patients With Pemphigus

Objective:To have a analysis and evaluation for the value of the anti epidermalintercellular desmosome antibody as well as Dsg1and Dsg3in the diagnosis for emphigus.Method:Use (Indirect Immunofluorescence) IIF to detect resistance to epidermalintercellular desmosome antibodies, use Enzyme Linked Immunosorbent Assay (ELISA)to detect Dsg1and Dsg3, count the sensitivity, specificity, negative predictive value andpositive predictive value of each antibody and make comprehensive evaluation andanalysis with the combination of the statistics and the clinical data.Result:Among34pemphigus patients and36matched group patients, the positive rates of anti epidermalintercellular desmosome antibody are respectively73.5%(25／34) and2.8%(0／20).While the positive rates of anti Dsg1antibody are respectively70.6%(24／34) and0.0%(0／36), the positive rates of anti Dsg3antibody are respectively79.4%(27／34) and5.6%(2／36). Among the pemphigus patients, the sensitivity of the resistance of theepidermal desmosome intercellular antibody is73.5%; Specificity:97.2%; PPV:96.2%,NPV:79.5%. The sensitivity of the anti Dsg1antibody:70.6%; Specificity:100%; PPV:100%, NPV:78.3%. The sensitivity of the anti Dsg3antibody:79.4%; Specificity:94.4%;PPV:93.1%, NPV:82.9%.There was no significant difference between IIF and Dsg1、3(P>0.05).Conclusion:The antibody detection in blood circulation and the confirmationtest of the specific target antigens can complement each other, reduce the clinicalmisdiagnosis and increase the diagnostic positive rate if the two tests were conducted atthe same time.The positive result of anti-pemphigus antibody is good at diagnosingpemphigus accurately, while the negative result is of great value to rule out pemphigus.