Isolation, Identification And Elementary Taxonomy Of Nail Fungi

In this thesis, history and present condition of onychomycosis have been introduced which including impressionable factors of onychomycosis , the method of getting specimens , diagnosis and identification of onychomycosis, composing and variance of pathogen and therapy of onychomycosis. At the same time , the epidemiology data's localization of present onychomycosis and the purpose and meaning of our study have been presented .In the first part of this research , isolation and purity method were introduced. Crumbs of nails were isolated from onychomycosis suffers, KOH was chosen to be solvent , nail fungi were examined by microscopy , cultured in Sabourud, purified by method of concentration grads and method of exsecting cusp of mycelia. Finally, sixteen purified strains were ready.In the second part, polyphasic taxonomy methods such as morphological method, physiological and biochemical method, DNA G+Cmol%, soluble protein electrophoresis and 18S rDNA sequence analysis were used for systematics of all strains isolated and purified with the method above.The results showed, Fh6 and Wj belong to Rhodotorula mudlaginosa and Rhodotorula sp, FJ, Xh3, F2, F1, FJ2,F8,FJ3 belong to Candida guilliermondii, Candida parapsilosis, Candida tropicalis and Candida albicans, W2 belongs to Cryptococcus neoformans, Fh2, Fh3, W3 belong to Cephalosporium acremonium and Cephalosporium sp, Fh5 belongs to Aspergillus flavus, Dh3 belongs to Acremonium, Fh4 belongs to Pleospora.In the last part of this research, elementary animal experiment were processed. Impressionable animals of superficial infected fungi are guinea pigs, but because of restrict of experiment condition, white rats were replaced. After inoculation, all strains were examined by microscopy, hyphae or cells of all strainswere observed, but none was seen in negative comparison. In the same time, originally fungi were isolated again in Sabourud which showed that these fungi could grow and reproduce in these animals, but if they can cause infection or not will be make sure with impressionable animals.Additional, according to the data, many superficial infected fungi don't arise obvious infection in skin of experimental animals, the status has become problem in animal experiments of nail fungi which await to further study.