| BACKGROUNDProblem of reproduction health of masculinity such as infertility, abortion, fetal deatrucongenital malformation has a tendency to be increased because of environmental pollution and ionization radiation in recent years. lonization radiation involves X-ray , neutro, obligation, a or (3 particle, y-ray. The most frequently that people contact is X-ray. An epidemiological study from abroad said that incidence of problem of reproduction health in man who are contact X-ray for a long-term is more higher than common people. So people pay close attention to how to reduce the incidence. Inhibin B is a serum markers of spermatogenesis. The level of inhibin B in normal man is 135. 6pg/ml . Assay of Inhibin B is not difficult. So we want to know whether inhibin B is a sensitive marker for reflecting the functional status of spermatogenesis in man who has a working in X-ray enviroment. As a locus of tricarboxylic acid cycle and oxidative phosphorylation, Mitochondrion is a main organell that provid ATP. Metabolism of cells can not procee in normal when mitochondrion are injuries. Mitochondrion is athe most sensitive organell for injuries. Observing the functional condition of mitochondrion through electron microscope can be used as a maker of earlier period injures. After exposure to a dosage of X-ray i n monkeys, testicle biopsy showed dilataltion of seminiferous tubule, significantly decreasing of quantity of spermatogenic cells, deformation of Sertoli's cells. Up to now, there is no study reported that inhibin B act as a maker to evaluate radioactivity injuries of spermatogenesis.OBJECTIVETo learn alteration of the function of mitochondria after exposure to X-ray and to explore the status and role of inhibinB on screening injuries of spermatogenesis in mice. MATERIAL AND METHODS10-12weeks age ,40 male mice were randomly divided into X-ray group and control group. Mice were given 2% soluble pentobarbitone 0.15ml through intraperitoneal injection .The dose of X-ray was 3.5Gy and was given mono-dosage. The mice were killed in batch in days of 2, 7, 21, 42 after X-ray exposure. Taking blood from eyeball and high speed centrifugation to obtain blood serum. Enzyme-linked immunoadsordent assay inhibin B and radio- Immunization assay the levels of FSH, LH, T. with dissection of testis and calculation the weight of testis. The sample for light microscope was fixed with 10% formaldehyde solution by turns with filleting and staining ; the sample for electron microscope was fixed with 2.5% glutaral by turns with filleting and staining. Observing structure of testis with light microscope and counting Sertoli's cell - interstitial cell and spermatogenic cell which include spermatogenous cell, spermatocyte, spermatid, Sperm. Observing the ultrastructure of spermatogenic cell and Sertoli's cell with electron microscope and pay attention to the function of mitochondrion at the same time. RESULTSTwo days after X-ray exposure, the weight of testis was not descent and no significant change was found by light microscope. But lesion of mitochondrion in spermatogenic cell and Sertoli's cell was found by electron microscope. The tite of inhibin B was descent at the same time(P<0.05). Seven days after X-ray exposure, the weight of testis was significantly descent(P<0.05). The quantity of spermatid and sperm was significantly decreasing(P<0.05). Lesion of mitochondrion in spermatogenic cell and Sertoli's cell was took a bad turn. Twenty-one days after X-ray exposure, the weight of testis continued significantly descent(P<0.05). The quantity of spermatogenous cell was slowly elevated, but others were still in a lower level. Rimmed vacuoles was found in cells through electron microscope. Disruption of cell membrane and outflow of organell. The quantityof sperm continued decreasing and more malformation sperm were found. Level of FSH was elevated. Forty-two days after X-ray exposure, comparing with the weight of twenty-one days, the weight of testis was no significant change(P>0.05). The quantity of Sperma...
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