Study Of Pulsing Electromagnetic Fields On Treatment Of Osteoporosis

Objective: With the development of Biomedical engineering, The treatment of osteoporosis in pulsing electromagnetic fields is attached more importance than ever and used in clinic because of the advantage of PEMFs in osteoporosis. In recent years many overseas research results show that PEMFs can prevent even reverse osteoporosis safely and effectively, it can increase the sum of bone without destroying the rebuilding of bone. Although we succeed in treating osteoporosis with PEMFs at present, the parameter of PEMFs we are using such as frequency, intensity, impulse is different, we cannot find the best parameter of PEMFs and cannot fully explain the effects of PEMFs.This experiment is to observe the effects of osteoporosis rats in different intensity and same frequency PEMFs . Through the experiment quantificationally analyze BMDs of experiment animals and histomorphometric measurement and calculations of distal femur and biomechanics characteristics and study the mechanism of PEMFs on prevention of osteoporosis. Through the experiment results makestatistic analysis and seek the best parameter for treatment.Methods:1 A PEMFs circuit was designed and improved. The outputs of PEMFs were sub-impulse groups with frequency of 15Hz and delay time of 5 ms through many experiments. The intensity of PEMFs could be continuously adjusted from 0 to 12 X 10-4T. This circuit had the function of timing. The diameter of winding was 100,150,200mm.2 The prevent experiment was started. Sixty-four female Sprague-dawley rats were divided randomly to 8 groups :normal control ; ovariectomized ; 2X10-4T PEMFs treatment ; 4X10-4T PEMFs treatment ; 8X10-4T PEMFs treatment; 4X10-4T PEMFs treatment at night ; drug treatment ;4 X 10-4T PEMFs and drug treatment. All groups except normal control group were ovariectomized. Then all groups were bred with the condition of different intensity for two months. BMD were measured by X-ray absooptionetry and histomorphometry on the bone were observed.3 The therapy experiment was started. Sixty-four female Sprague-dawley rats were divided randomly to 8 groups :normal control ; ovariectomized ; 2X10-4T PEMFs treatment ; 4X10-4T PEMFs treatment ; 8X10-4T PEMFs treatment; 10X10-4T PEMFs treatment; 4X10-4T PEMFs treatment at night; drug treatment. All groups except normal control group were ovariectomizedAU those were bred normally for two weeks and then were bred with the condition of different intensity for two months. BMD were measured by X-ray absooptionetry and histomorphometry on the bone were observed.Results:1 After all the prevent group were irradiated two months, all rats were made death .Compared with normal control group, BMDs of ovariectomized group failed significantly and the microstructuresalso pathologically changed. In all different intensity PEMFs BMDs of rats and histomorphometric measurement and calculations of distal femur and biomechanics characteristics were all not changed significantly compared with normal control group. The differences between normal control group and 8X10-4T PEMFs treatment group ,drug treatment group or 4X 10-4T PEMFs and drug treatment group were not more significant than 2X10-4T PEMFs treatment group, 4 X10-4T PEMFs treatment and 4 X 10-4T PEMFs treatment at night group. Through the pathological slices of bones, we could see that 8 X 10-4T PEMFs treatment group ,drug treatment group or 4 X 10-4T PEMFs and drug treatment group incrased more little girder , arranged more orderly and joined together to nets than other groups ,and the marrow areas of these groups decreased more significantly than other groups too.2 After ovariectomized, BMDs of all therapy group failed significantly and the microstructures also pathological changed. Through two months irradiation, in all different intensity PEMFs BMDs of rats and histomorphometric measurement and calculations of distal femur and biomechanics characteristics were all changed significantly compared with ovariectomized group. The differences between ovariectomized group and 8 X...