| Prostaglandin E1(PGE1) , as a kind of vasodilator and anti-platelet aggregation drug, has shown a fairly good effect in the curing Pulmonary Hypertension caused by many reasons. Also it has been reported recently that PGE1 has protective functions in Myocardial Ischemia and Reperfusion Injury ( MIRI) . Lipo-PGE1 can be defined that by exploiting the characteristics of Lipo-Particle, PGE1 is sealed into 0. 2m-Lipo-particle so as to obtain less inactivity of the drug in the lung and reduced stimulation of drug to vascular wall. In order to explore a new method to myocardial preservation in clinical application, this study is based on experiments to investigate the effect of combination of Lipo-PGE, with cardioplegic solution and combination Lipo-PGE1 with Krebs-Henseleit solution (K-H solution) on MIRI and thereafter to identify whether Lipo-PGE1 can prevent MIRI effectively and also to investigate cardioprotective mechanism by using methods as following: firstly combining the Lipo-PGE, with cardioplegic solution; secondly combining the Lipo-PGEj with K-H solution, respectively; then by employing the Langendorff apparatus on the isolated mouse hearts to create a model of MIRI and concurrently by recording the change of hemodynamics and coronary flow(CF) , lastly measuring superoxide dismutase(SOD) , malonalde-hyde(MDA), Adenosine triphosphate (ATP), lactic dehydrogeriase ( LDH) and Creatin phosphokinase(CPK) , observing myocardial pathological change by electron microscope.Methods1. the standard of dispensation of K-H solution (mmol/L)NaCl: 118.5; NaHC03: 25.0; K-H2P04: 1.2; KCL:4.8; MgS04 7H2 O:1.2; CaCl2 7H20:1. 8; Glucose: 11. 0. Weighted by analytical balance firstly, then dissolved in distilled water (osmotic pressur 324mosm/L) , followed by being equilibrated with 95%O2and 5% C02 (PH 7.4-7. 5, PC02 32 4mmHg, P02642 17mmHg) , finally, filtering the granules by 4. 5n filter paper.2. Langendorff apparatusThe used experimental device, made by the extracorporeal circulation department of FU WAI Cardiovascular Hospital, is a double layers wide opening glass bottle connected with thermostatic -water pump. There are two holes on the cork of the bottle, aortic cannlation is inserted into one hole and thermometer is inserted into another. Another end of the aortic cannlation is connected with car-dioplegic solution, heart perfusate, 60cm controlling pressure tube, respective-ly.3. Establishing model of perfusing heart by langendorff apparatus Applying the langendorff device in experiment; firstly, injecting pentobar-bital (30mg/kg) into abdominal cavity of experimental mouse, then, injecting hepalean (200U) into femoral vein of the mouse, thirdly, excising the heart of the mouse,immersed in solution of 4 K-H solution, then mounting the heart on the stainless-cannula of a modified Langendorff perfusion apparatus and perfusing K-H solution though aorta to eliminate the residual blood of the coronary artery (perfusion pressure 5.88kpa) , fourthly, scissoring pulmonary artery radical open to make the coronary circulate freely, Fourthly, scissoring left auricle and to put the latex sacculus which is connected to multipurpose polygraph into left ventricle by crossing the left auricle and placing the isolated heart into thermostatic glass bottle, finally injecting saline into sacculus slowly while adjust the press of the sacculus in order to keep left ventricular end-diastolic pressure (LV-EDP) to reach up to l0mmHg around.4. Establishing model of heart ischemia/reperfusionThe process of all groups of heart ischemia/reperfusion as following: firstly, perfusing K-H solution into heart by aorta so as to make the heart re-beaten and last 20 minutes, then perfusing 20ml of ST Thomas solution into heart by a-orta within 5 minutes so as to make the heart stop beating, thirdly, placing the heart into 25 saline lasting 50 minutes, finally, reperfusing K-H solution so as to make the heart re-beaten and making it last 60 minutes.5. Divided experimental groups30 S-D male mice w...
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