Research On Lipo-PGE1to Protect Ischemia Reperfusion Myocardial Injury And The Effects Of Lipo-PGE1on Blood Rheology And Thromboxane B2of The Patients With Angina Pectoris

Objective To observe the protective effects of Lipo-PGE Ion coronary ischemia reperfusion injury in rats and to explore the effects of Lipo-PGE lonleft ventricular function, blood rheology and thromboxane B2of patients with unstable angina.Methods1.24experiments with rats, divided randomly into4groups with6in each group: Ischemia group; ischemia reperfusion groups:including not to medicine reperfusion group. Low doses of PGE1(2ug//ml/kg) for reperfusion groupand high dose PGE1(6ug//ml/kg) for reperfusion group. To medicine at5minutes before reperfusion2hours after ischemia. To determine each animal’s plasma endothelin1(ET1), serum malondialdehyde (MDA). superoxide dismutase (SOD) after0.5h,2h,4h of reperfusion respectivelyto determine.2.150patients with unstable angina were divided randomly into conventional treatment group with75cases (control group) and the PGEltreatment group with75cases (treatment group), Treatment group:on the basis of conventional treatment combined with PGE110ug, once per day, intravenous drip, the patients in two groups all receive the treatment for2weeks. To determine left ventricular function, blood rheology indexes and plasma thromboxane B2(TXB2) level before and after the treatment in two groups,, at the same time record episodes of angina.Results1.Coronary ischemia for2hours rats group compared with reperfusion group2hours after ischemia, MDA increased slightly (25.35vs32.24, P>0.05), SOD decreased slightly (113.9vs109.98, P>0.05), ET-1increased significantly (133.29vs169.69, P<0.01), After application of PGE1, dosage group compared with not give medicine group, the MDA decreased (34.56vs9.25, P<0.01), SOD increased (102.56vs174.33, P<0.01), ET-1decreased (182.56vs104.13, P<0.01). All the indicators of high dose group changed most obviously (P<0.01).2.Conventional treatment+Lipo-PGE1treatment, can further improve the clinical symptoms of patients with unstable angina, reduce levels of plasma TXB2(2.65vs1.42, P<0.01), improve left ventricular function (LVEF increase,47.48%vs59.03%, P<0.05), reduce blood viscosity. each index was improved after treatment (P<0.05or<0.01), but they improved more significantly in treatment group compared with control group (P<0.05or<0.01).Conclusion1.2hours after ischemia-reperfusion injury, it was not obvious that MDA increased (P>0.05)and SOD decreased (P>0.05), suggested that lipid peroxidation damage reaction was not significant.2. After the application of PGE1, MDA and ET-1were decreased significantly, suggested that the change is inversely proportional to the dose of PGE1, PGE1can reduce lipid peroxidation damage response.3. In patients with coronary heart disease angina pectoris, on the basis of conventional treatment combined with PGE1, can further reduce TXB2level and blood viscosity, reduce attack frequency of angina pectoris and improve left ventricular function.