| There is no up - to - date breaking progress on tendon adhesion after surgical repair. During the course of tendon repair, the intrinsic healing and the extrinsic healing are favourable, but adhesion caused by the extrinsic healing is a major factor affecting tendons function. Therefore, it is a better way to prevent or decrease adhesion that some suitable measures are applied to make the tendon regeneration inside faster than the proliferation of peritendinous connective tissues. Basic fibroblast growth factor (bFGF) can stimulate differentiation of fi-broblasts and accelerate synthesis of collagen fibrils. In the early stage of tendon healing, injection of bFGF in local can stimulate cell proliferation and collagen expression, suggesting that bFGF plays an important role in the course of tendon healing. Based on the above opinion, bFGF composite degradation membrane is encapsulated to the tendon autografts location in order to accelerate the tendon regeneration inside and make it faster than the proliferation of peritendinous connective tissues so that adhesion can be avoided.Methods1. Animals and grouping:60 Newzerland rabbits are divided into A and B groups randomly by means. The tendon autografts are sutured to the 2. 0 cm defects in the profound flexor digitorium tendons of the 2nd toe on the left anterior limb. The group A is the experimental group in which the tendon autograft is encapsulated with the bFGF composite degradation membrane. The group B is the control group in which thetendon autograft is not encapsulated with the bFGF composite degradation membrane.2. bFGF composite degradation membrane:The bFGF composite degradation membrane includes two major ingredients of bFGF and vitamin C.3. Observation contents:The observation contents include:(1) Macroscopic evaluation;(2) Histological evaluation;(1)H. E. staining: to observe nuclei of fibroblasts(2) Mallory staining: to observe collagen fibrils (3) AgNO3 staining: to test the concentration of vitamin C (4) Electron microscope sample: to observe the ultramicrostructure of fibro-blasts and collagen fibrils( 3) Measurement of the quantity of fibroblasts and collagen fibrils by Luzex - F Image Analyzer(4) Analysis of the adhesion around tendon autografts ( 5 ) Determination of hydroxylproline content (6) Biomechanical property assayResults1. Macroscopic evaluation:In the experimental group ( A group) , tendon grafts show features such as slight enlargement, smooth surface, fine gliding and slight loose adhesion with tissues around; In the control group ( B group) , tendon grafts show features such as obvious enlargement, rough surface, immobilize gliding and finned adhesion with tissues around.2. Histological evaluation: 2.1 Light microscope { LM) :The AgN03 staining grains of vitamin C in the experimental group ( A group) are much more than those in the control group (B group) ; In the experi-mental group ( A group) , the fibroblast nuclei and collagen fibrils inside the tendon autografts are much more than those around the tendon autografts, the boundary between tendon autografts and peritendinous tissues is distinct; In the control group ( B group) , the fibroblast nuclei and collagen fibrils inside the tendon autografts are much fewer than those around the tendon autografts, the boundary between tendon autografts and peritendinous tissues is blurred; The fibroblast nuclei and collagen fibrils inside the tendon autografts in the experimental group ( A group) are much more than those in the control group ( B group).2.2 Electron microscope (EM) :At 8 weeks after surgery, fibroblasts have active function structures and collagen fibrils are thin and disordered in the experimental group ( A group) ; At 24 weeks after surgery, unactive fibroblasts increase and collagen fibrils are thick and ordered in the experimental group (A group). These structural changes in the experimental group ( A group) are obviously much better than those in the control group (B group).3...
|
PDF Full Text Request