| Objective: The traumaed tendon needs the pretty long time ,therefore it often longtime outside fixation to prevent tendon from breaking again. But longtime fixation makes the effective skill function having no way to carry out in time and the tendon aggravated and affect the renovation effect that tendon injures gravely. It's very important to adopt various measure boosting tendon healing. The bFGF(basic fibroblast growth factor, bFGF) has the promoter action to the organization repair. It recives the widespread attenion. Our initial period findings and so on Chang indicated that in initial period the experssion of bFGF insufficiency on tendon healing in the sheath. Therefore, we use exogenetic bFGF in the end of tendon in oder to discuss the fuction of exogenetic (basic fibroblast growth factor, bFGF) in renovation and adhesion.Methods:Divide 72 health male Harberd chicken(4-5kg) into 3 groups randomly. The experimental animal by intramuscular injection anaesthesia with the ketamine (30 mg/kg) was fixed properly in the control desk. In aseptic condition, professional jargon was selected underneath chicken right knee joint. Then the flexor digitorum profundus tendon was confirmed and was cut off. Professional jargon as"┖┚"shape was selected at the third dactyl of the right foot to separate the tela subcutanea and cut open the sheath tube between the A2-A4 pulley, to confirmed the flexor digitorum profundus tendon and cut off. The model of flexor digitorum profundus tendon transsection of the third dactyl was prepared.Group A: The tendon cut off was sutured by 6/0 non-wound partition suture in the home position. The suture method was Improved Kessler and the adventitia of four situation was sutured. Then the tendon sheath and the skin were sutured. The claw was fixed by plaster as boxing glove shape.Group B: The tendon cut off was sutured by 6/0 non-wound partition suture in the home position. The suture method was Improved Kessler and the adventitia of four situation was sutured. A miniature tube was put in the sheath tube. The air in the tube was evacuated. The tube was fixed on the surface of the third dactyl to prevent the tube slipping. The skin was sutured. The claw was fixed by plaster as boxing glove shape. The experimental animal was used 5μl bFGF by the tube after 24h of operation,1/d.The tube was demolished after 3 days.Group C: A miniature tube was put in the broken ends of tendon. The air in the tube was evacuated. The tendon cut off was sutured by 6/0 non-wound partition suture in the home position. The suture method was Improved Kessler and the adventitia of four situation was sutured. The tube was fixed on the surface of the third dactyl to prevent the tube slipping. The skin was sutured. The claw was fixed by plaster as boxing glove shape. The experimental animal was used 2μl bFGF after 24h of operation,1/d.The tube was demolished after 3 days.The experimental animal of each group was used 40ku Gentamicin by intramuscular injection,1/d,3d,in order to prevent infection. The plaster fixation was dismantled after 1 week, then the experimental animal can arbitrarily exercise. The tendon of the third dactyl of the right foot of 8 animal of each group was experimented at gross appearance and biomechanics determination.Results: In the tendon concrescence situation, the concrescence of B and C two group was similar, and more quickly than group A, and the stretching resistance intensity is stronger. In the adhesion situation, the adhesion degree of group B was more serious, the adhesion degree of A and C two group was similar.1 Gross appearance: After 2 weeks of operation, the wound healing of each group was good, the hypodermis and tendon were light degree dropsy with small amounts of synovia. There was 1.5cm crevice in broken ends with rubber peptone type organization that forms the spindle-shaped tubercle and with the peritonsillar tissue loose adhesion. Compared with group A, the synovial fluids of B and C two groups seep out few, the rubber peptone type organization transparency is also bad. But with the A and C two groups compares, adhesion of group B is more widespread. After 3 weeks of operation, the B and C two groups have the mild adhesion, but easy to separate. There was a few synovial fluids in the tendon sheath. The tenacity of the end rubber peptone type of the tendon broke compared before increased obviously. The spindle-shaped tubercle increased and was more tenacious, but the gap of breaks of the end did not increase. Compared with group B and C, the rubber peptone type material of group A breaks between the end did not increase obviously, but the gap of breaks of the end did increase slightly. But Compared with group A and C , the tendon adhesion scope of group B became broad slightly. After 6 weeks of operation, the spindle-shaped tubercle reduced. The color bleached, and the tenacity increased. The glide performance of group A and C became better than before. The adhesion of group B was more serious than group A and C. Table 1 showed the adhesion grade after 2, 3, 6 weeks operation.2 Biomechanics results: In order to reduce the individual the difference influence, extracts tests the foot with the respective opposite side normal foot's ratio, then the correlative value carries on statistics processing.2.1 the flex angle of the joint: Table 3 shows the flex angle of the joint of the testing toe and the normal toe. The statistical result shows regardless at 2, 3 or at 6 weeks, the difference of joint flexure angle ratio of each group was significant (P<0.05). Further each comparison shows that the joint flexure angle ratio of group A,C is greater than group B, the difference is significant(P<0.05).While the difference between group A and group C is not significant(P>0.05).2.2 Biomechanics determination of tendon adhesion: Table 4 shows the biggest strength which pulls the tendon from the sheath tube. Carrying on the multi-samples to compare, the adhesion of group B was more serious than group A and C, the difference is significant(P<0.05).While the difference between group A and group C is not significant(P>0.05).2.3 The breaking strength of the tendon: The break spot of each group experiment foot was the kissed place. Table 3 shows the breaking strength of the tendon. The statistical result shows regardless at 2,3 or at 6 weeks, the difference of the breaking strength of each group was significant (P<0.05). Further each comparison shows that the breaking strength of group B,C is greater than group A, the difference is significant(P<0.05).While the difference between group B and group C is not significant(P>0.05).2.4 The difference between the breaking strength of the tendon and the biggest strength which pulls the tendon from the sheath tube: Table 5 shows the difference between the breaking strength of the tendon and the biggest strength which pulls the tendon from the sheath tube. The statistical result shows the difference was significant (P<0.05). Further each comparison shows that the difference of group C is greater than group A and B, the difference is significant(P<0.05).While the difference between group A and group B is not significant(P>0.05). Conclusion:Setting the tube short-term to apply bFGF (basic fibroblast growth factor, bFGF) to breaks between the end can promote the tendon cicatrization effectively. It provides a new method to reduce the tendon adhesion degree by early function exercise .
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