An Experimental Study On BFGF Combined Slow-releasing Degradatable Membrane Which Is Placed Around The Injured And Sutured Tendon

BackgroundIt is always a difficult problem in clinical that the adhesion always occurs when the tendon is injured. Researchers of all over the world try to resolve this problem by many methods ,but they have not achieve a great progress. Now, people believe that tendon have intrinsic and extrinsic healing, and it may be the proliferation of the peripheral connective tissue which cause the adhesion. Thus, our experiment try to prevent adhesion in the tendon healing by the combined slow - releasing degradatable membrane . The membrane consists of bFGF, which can increase the amount of fibroblast inside the tendon, and the agents, which can improve collagen fibril synthesis.ObjectiveTo study the adhesion - preventing effect in the tendon healing by the combined slow - releasing degradatable membrane which consists of bFGF, which can increase the amount of fibroblast inside the tendon, and the agents, which can improve collagen fibrils synthesis.MethodsThe bFGF combined slow - releasing degradatable membrane is madefrom bFGF and the agents which can promote collagen fibrils synthesis . Sixty - six SD rats , weight 450 550g( achieved from CMU) , were divided into A, B, C three groups randomly with each group contains twenty -two rats . After anesthesia, the achilles tendon of the left posterior limb is incised , and sutured with approved Kessler method. Group A : sutured a-chilles tendon were encapsulated with bFGF combined slow - releasing degradatable membrane ; Group B : sutured achilles tendon were encapsulated with degradatable membrane without any agents; Group C : achilles tendon were only sutured. After operation, the rats were fixed with plaster cast and given routine therapy. Ninety days later, light - microscope , electron - microscope, figure - analysing , hydroxyproline content, extent of peritendon adhesion and biomechanic test were evaluated.Results(1)It was shown on the HE, Mallory and AgNO₃ stained specimen that the a-mount of fibroblast , collagen fibrils and the vitamin - C particles inside the sutured tendon is larger than that of peripheral connective tissue and group B and C ( p <0. 05 ). Under the electron - microscope, the amount of excellent fibroblast and collagen fibrilla in group A is larger than that of the B and C. The content of hydroxyproline and the ultermost antihauling intensity of group A is higher than that of group B and C. (2) The peripheral tissue in group A almost remains the formal loose connective tissue and it seldom grows into the tendon. There is a clear border between the tendon and the peripheral tissue. But, it becomes dense connective tissue in group B and C , and it grows into the tendon. The amount of nucleus of fibroblast and the collagen fibrilla in peripheral tissue is larger than that in the tendon (3) Moreover, the extent of adhesion in group A is lesser than the other two groups according to the mensuration of peritendon adhesion (P < 0.01).