| Indomethacin is a kind of NSAIDS anti-inflammation and anti-immune medicine, which has good analgesic effect using for decreasing rheumatic disease and Arthritis for a long time. Recently there are so many experiments and clinical observings showed that IN can preclude the symptom causing by tumor, reduce pain, control fever and losing weight causing by tumor, Enhance the therapy to tumor. In our experiment we use H-IN as a tracer and give it to the C57 mice bearing tumor. Then we use ARG and liquid scintillation counting techniques to observe 3H-IN distribution in vivo and if IN had the potential concentration to the tumor tissue selectively.MaterialsFemale C57BL/6 inbred line mice and C57BL/6 bearing Lewis lung cancer The Hydrogen 3 Labelled indomethacin Scintillation liquid Beckmen autoradiometeAtomic nucleus liquid lacteprene, developer solution (D-19b) , fixing solution (F-5) , lacteprene smear box are needed.MethodsAnimal groups: After number all the animals , divide the normal control 25 C57mice and the 25 C57 mice bearing tumor into 5 groups differently . The 5 groups are divided according to the time of submiting IN. They are 2. 5h,4h,7h,12h,24h. Scintillati on Measurement.Every mouse form normal control group and bearing tumor group was administered 3H-IN liquid 0. 2ml containing IN 125g, radioactivity 185KBq. Then treat the mice at 2.5h,4h,7h,12h,24h after giving the medicine.Firstly get the mouse' s blood, collect about 5 drops blood on one piece of filter paper whose weight was already known. Then measure it cpm After that kill the mouse, get the liver, kidney, muscle, fat and tumor tissue then weight and homogenate each one, get the cpm Finally.ARG slices; make the tumor tissue into pathological section%t 7m. Under the safe red light in dark room, in the lacteprene smear box , smear over with liquid, dry in the air then place in the dark box, add the dry bag sealed completely, placed in the refrigerator at 4 expose for 21 days .after exposure place the slides into developer(19 1) D-19s 4min, well water washed, then put them into the fixing solution F-5 l0min, finally the HE sealed after the slices dying.All the results of measurement data express as x s, two samples mean Compared with t test. Size of test P =0.01, P <0.05 stand for significant, P < 0.01 stand for extremely significantResults3H-IN had rapid blood clearance and accumulated in tumor tissue withhigher levels of radioactivity than that in others tissues. At 12h the quantity ofradioactivity of tumor is significantly higher than that of blood , muscle and fat( P<0.01). At 4h tumor-to-muscle, tumor-to-fat, and tumor-to-blood ratios >1and with the time past they reached the peak at 12h.The results of ARG showed that there are particles of sliver in the cell's plasm and nucleus at the same time , and the quantity in the plasm was larger than that in the nucleus.DiscussionIn 1973 Stoll had found that indomethacin could contral tumor growing and make the animals live longer. Giving IN to the patient with mammary cancer (100-150mg) , after two weeks the transfer tumor nod on chest wall (0. 5-1. Ocm) disappeared. Al-Salem reported there were 9 patients with squamous carcinoma using IN(25-100mg) , after three weeks finding that one of them the mass was abolishment Panje gave IN (75-100mg) to 7 patients with head and neck malignant tumor, after few weeks the tumor was limitated by differendy degrees. Besides that IN is also positive affect on lung cancer, esophageal carcinoma, intesting tumor, liver cancer and so on. The animal experiments indicated that taking IN (6. 25mg/kg) one time per two days could limited the LEWIS lung cancer growing. Furthermore IN produced a marked effect during anti-tumor colligation therapies.The tumor epidemic, clinical research and animal experiment all show that the IN has the obvious anti-tumor function, its mechanism may be that IN have the cell toxicity function, killing the tumor cell directly. Or depressing excretion the prostaglandin E2, st...
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