| INTRODUCTIONProtease and its inhibitor play an important role in the process of tumor incursions and metastasis. Extracellular matrix can be degraded by protease, but blocked by protease inhibitor. Recent findings reveal that maspin as a member of serpin family has tumor - suppressive activity, which has been proved in mammary cancer, carcinoma of prostate, etc. Researches show that maspin inhibits the process of incursion and metastasis through blocking the movement and proliferation of tumor cells.Expression of maspin is different in most human tissues, while has a consistent decrease in the development of tumor. Recent reports demonstrated that maspin was effective in inhibiting angiogenesis in vivo and in vitro, so as to decline the density of microvessels and inhibit tumor. Revealed by new evidence, there was an interaction between maspin and p53 in the inhibition of tumor. In partial tumors, the expression of maspin was negatively associated with the level of mutant p53 protein, indicating that maspin might be a target gene for p53. Maspin protein expressed highly in mammary gland, prostatic gland, colon and oral squamous tissues, etc. , but the expression of maspin had a tendency to decrease in corresponding tumor tissues, and were correlated with more malignant and bigger tumor, higher histological grade, lymphnode metastasis, local relapse and progress of tumor, short survival period and positive mutant p53 protein expression. It was in accordance with the function to inhibit tumor. Other researches revealed that the expression of maspin protein was abnormal in pancreatic carcinoma and ovarian carcinoma, i. e. the expression was decreased ordeficient in normal tissues, while increased in corresponding cancer tissues. There were two opposite opinions on the expression of maspin in the gastric carcinoma. Nicolar Maass, et al. reported that there was no maspin mRNA in 6 cell lines of gastric carcinoma, but reported by Son HJ, et al. the expression of maspin was higher in intestinal metaplasia and gastric carcinoma than non - cancerous mucosa, in agreement with the expression of mRNA. As yet, reports on the expression of maspin in gastric carcinoma and studies on the correlation between the expression of maspin and p53 are rare. In the study, immunohisto-chemical method was performed to detect the expressions of maspin and mutant p53 protein, analyze the correlation of maspin protein expression with pathological typing, staging of gastric carcinoma, lymphnode metastasis and the expression of mutant p53 protein so as to investigate its biological significance and related molecular pathological mechanism in the carcinogenesis and progress of gastric carcinoma.MATERIALS AND METHODS1 Clinical dataTotally 156 paraffin samples of gastric carcinoma were selected from the Cancer Institute, No. 1 Hospital of China Medical University and Iiaoning Province Cancer Hospital, including 45 cases of intestinal - type gastric carcinoma, 111 cases of diffuse - type gastric carcinoma, 20 cases of papillary adenocarci-noma, 2 cases of well - differentiated tubular adenocarcinoma, 23 cases of moderately differentiated adenocarcinoma, 74 cases of poorly differentiated adenocarcinoma, 9 cases of undifferentiated carcinoma, 21 cases of signet -ring cell carcinoma, 6 cases of mucinous adenocarcinoma, and 1 cases of carcinoid. There were 32 cases of early gastric carcinoma, 28 of metaphase gastric carcinoma and 96 of late phase gastric carcinoma, among which, lymphnode metastasis was found in 106. None of patients received chemiotherapy or radiotherapy before operation. Complete clinical data and follow - up data were prepared. The median age was 49 years old (30-80 years old).2 Methods and immunostaining evaluationExpressions of maspin protein ( mouse anti - human maspin monoclonal antibody, working concentration; 1 : 50, Novocastra Co. ) and p53 protein (mouse anti - human p53 polyclonal antibody, working concentration; 1 : 100, Zymed Co. ) were assessed with immunohistochemical me...
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