Clinical Pathology Of Maspin,P27,P53 Expression And Colorectal Cancer

Object:By retrospective case analysis of 62 cases of colon cancer in Shandong Province, Zibo Central Hospital,, maspin, P27, P53 expression in colon cancer, a clear maspin, P27, P53 and clinical features of colon cancer, and the mechanism of action, seek colon cancer the occurrence and development mechanism, as well as explore the maspin, P27, P53 in the diagnosis and treatment of colon cancer.Medium:Select 62 cases of gastrointestinal surgery colon cancer patients of Zibo Central Hospital, Shandong Province,, while 30 patients selected colon polyps and 20 cases of normal colon mucosa as a control, Using immunohistochemical method to detect the expression of maspin, P27, P53 in colon cancer, colon polyps and normal colonic mucosa.62 cases of colon cancer patients for clinical pathology statistics and case follow-up, using statistical retrospective analysis, research maspin, P27, P53 expression and colon age, sex, tumor location, tumor size, depth of invasion, lymph node metastasis, organizational relationship between the types of pathology, and preliminary determination contact maspin, P27, P53 between immunohistochemical results based on a preliminary analysis of the mechanism of maspin in the development of colon cancer. Statistical SPSS13.0 statistical software for statistical analysis, count data using the χ2 test, p<0.05 was considered statistically significant, the standard test for α=0.05.Results:Analysis results to determine:a randomized double-blind, asked the two clinical pathologists in the case of unknown pathological state read the piece. Fine particles appear brown cells or tissues for immunohistochemistry positive. Observe the sections at 40 times the high power microscope. According positive cells and color intensity of positive cells grade score, adding two, and then determine the positive strength. Maspin protein immunohistochemistry coloring is mainly cytoplasmic location, color or both in the cytoplasm and the nucleus, most diffuse, the color was brown. Maspin protein in colon cancer cells, adenomatous polyp and normal colonic mucosa epithelium were expressed. P27 mainly in colon tumor cell nuclei coloring:colorectal adenoma and normal tissue P27 positive reaction products were mainly located in the mucosal epithelial cell nuclei, grainy, color brown. P53 is mainly expressed in colorectal adenoma and colorectal cancer cells and normal tissue glandular epithelial cells。In 62 cases of colon cancer maspin, P27, P53, positive expression rates were 17.7%,25.8%,59.7%. In 30 cases of colon polyps maspin, P27, P53 positive expression rate was 86.7%,90.0%,33.3%.20 cases of normal colon mucosa maspin, P27, P53 positive expression rates were 90.0%,95.0%,15.0%. Statistical analysis showed that the expression of maspin, P27, P53 in colorectal cancer tissues were significantly different, Maspin, P27 expression in colon cancer missing, The P53 protein over expressed. Which maspin expression rate of colon cancer in patients with tumor size, tumor stage, depth of invasion, lymph node metastasis, differentiation was negatively correlated(p<0.05). P27 expression rate of colon cancer in patients with tumor size, tumor stage, depth of invasion, lymph node metastasis, differentiation was negatively correlated(p<0.05). Tumor size P53 protein expression in patients with colon cancer, tumor stage, depth of invasion, lymph node metastasis, differentiation is positively correlated(p<0.05).