The Experimental Study On Part Of Vasoactive Substances Following Acute Pulmonary Thromboembolism In Dogs

The endothelin(ET), nitric oxide(NO), adrenomedulin(ADM) and C-type natriuretic peptide(CNP) are four types of vasoactive substances , mainly deriving from endothelium . ET and NO are respectively the strongest vasoconstriction and vasodilatation known , ADM and CNP also play a role in vasodilatation. But it is unkonwn if they are involved in pathophysiological course of acute pulmonary thromboembolism (APTE) . Objective To investigate the changes of ET, NO, ADM and CNP with APTE . Methods 14 healthy canines were randomly divided into two groups: pulmonary thromboembolism(PTE) group and non-PTE one as control. All the canines received conventional mechanism ventilation. Thrombus of oneself body was injected to the right jugular vein of the canines in the PTE group, while in the control group warm saline was injected. The hemodynamics markers were recorded and arterial blood were collected at the time of before PTE and 0.5h, 1h, 2h, 4h and 6h after PTE for blood gas analysis, the level of ET, NO, ADM and CNP. Resu I ts Blood gases profile:After the clots injection, PaO2 immediately decreased significantry(P<0.05). Hemodynamic profile: MPAP of the PTE group remained elevated after PTE (P<0.05). The level of vasoactive substance: The level of ET and NO increased significantly until at 2h, 4h, 6h after the injection of clots (P<0.05).The elevated level of AND in the PTE group present a striking contrast to the control at lh,2h (P<0.05)after PTE. The plasma concentration of CNP increased significantly at 2h after PTE(P<0.05).Conc I us i on We succeeded in making the model of PTE with thrombus of oneself body .The data suggest that the four kinds of vasoactive substance may be involved in pathophysiological course of APTE.