Expression Of Prolyl-4-hydroxylase And Heat Shock Protein 90 Genes In Placenta Of Patients With Pregnancy Induced Hypertension And Their Clinical Significance

Pregnancy-induced hypertension (PIH) is a pregnancy specific disease that characterize as hypertension, proteinuria and edema. Severe PIH can be complicated with intra-uterine fetus death and maternal death due to cerebral haemorrhage,renal and heptic failure. Various factors involve etiology and pathogenesis of PIH, including uterus-placenta ischemia, vascular endothelial dysfunction, immune maladaptation and genetic disease.However, its etiology remains elucidated. It is well known that placenta play a major role in PIH.Objective: To investigate the expression of prolyl-4-hydroxylase (P4H) and heat shock protein 90 (HSP90) in placenta from PIH and normal pregnancyMethods: Semi-quantitative RT-PCR was used to detect the mRNA levels of P4Hand HSP90 in placental tissue of PIH and normal pregnancy.Results: 1 Both PIH placenta and placenta from normal pregnancy express P4H, but the mRNA level of P4H was significantly higher in PIH placenta than that from normal pregnancy (p=0.037) .2 HSP90 is expressed in placental tissues of PIH and normal pregnancy.No difference was found between PIH and normal pregnancy in termHSP90(p=0.634).Conclusions: 1 The expression of P4H in PIH placenta is upregulated,suggesting that this gene may involve the pathogenesis of PIH.2 Placenta either from PIH or from normal pregnancy express HSP90. The expression level is comparable between PIH and normal pregnancy.