The Study Of Differential Proteins In Placenta Tissue Of Pregnancy Induced Hypertension Using Two-dimensional Polyacrylamide Gel Electrophoresis

Pregnancy-induced hypertension (PIH) is a disease which is specially related with pregnancy. The syndrome includes hypertension , edema and proteinuria. In China, the mobidity of PIH is 9.4-10.4%. PIH account for 20% -25 % of maternal mortality. The etiology of this disease remains unknown. Immunal factors, genetic factors, ischemia of placenta and endothelial dysfunction are suggested to associate with the pathogenesis of PIH.Objective to investigate the differential proteins of placenta tissue from pregnancy induced hypertension.Methods Two-dimensional polyacrylamide gel electrophoresis (O'Farrell system and immobilized pH gradients) was applied to display the total proteinsfrom placenta of 10 patients with PIH and 10 healthy pregnancy women. Differential proteins were distiguished by image analysis of the two-dimensional gel images. Mass spectrometry fingerprint analysis was used to identify differential proteins.Results Sixteen differential proteins were identified form PIH placenta. Two proteins were further analyzed by mass spectrometry fingerprint analysis. Results of the analysis show that one differential protein was Phospholipase C -2 (PLC-2) and the other was a unknown protein.Conclusion Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry analysis can be used to study pathogenesis of PIH. The signal transmission mediated by PLC may play some role in pathogenesis of PIH.