Study On The Effect Of Passive Immunization Of Hepatitis B Immunoglobulin Blocking The Maternal-infantile Vertical Transmission Action

Objective: To study on the effect of hepatitis B immunoglobulin(HBIG) on the blocking of maternal-infantile vertical transmission. Methods: 46 cases of hepatitis B virus positive pregnant women were divided into two groups. In the experiment group, 30 cases were injected with HBIG at 28?32?36 week during pregnancy (200 IU per time), it includes 8 cases that were HBsAg, HBeAg positive and 22 cases that were HBsAg positive. In the control group, 16 cases were followed-up without injection of HBIG, it includes 4 cases of HBsAg, HBeAg positive and 12 cases of HBsAg positive. The fetal HBV infection rate and placenta tissues HBV infection degree were compared according to different blood serum model. By using enzyme linked immunosorbant assay (ELISA) and fluorescence quantitative polymerase chain reaction (FQ - PCR), the HBV marker (HBVM) and HBVDNA load in the blood samples of mothers and their neonates were tested respectively. The immunohistochemistry Power Vision? PV9000 assay was used todetect the HBV infection degree of placenta tissues. Results: (1)Two groups ofplacenta tissues that were bom by HBsAg, HBeAg positive pregnant women were all HBV infected. In HBIG experiment group, the mean density of positive cells (brown stained cells per mm2) is 16.36?.97/mm2 , it was significantly lower than that in control group (24.97?.11/mm2)(t=4.27, p<0.01).(2)Another two groups of placenta tissues that were bom by HBsAg positive pregnant women were detected. In HBIG experiment group, the mean density of positive cells in HBV infected placentas is 6.85?.39/mm2 , it was significantly lower than that in control group (9.68?.18/mm2)( t=3.12, P<0.01). (3)In experiment group, there were 15 infants and 16 placentas that were HBV infected; in control group there were 10 infants and 10 placentas that were HBV infected. There wasn't any difference between the twogroups( x2=0.66?0.36, P>0.05). Conclusion: The HBV infection degree on placenta tissues that were bom by HBV positive pregnant women who had accepted HBIG passive immunization can be reduced significantly ,but the fetal intrauterine HBV infection rate and placenta tissues HBV infection rate can not be reduced significantly.Liu Shaohua (Department of Obstetrics and Gynecology) Directed by Dai Shuzhen(professor), Hao Cuifang (Associate professor)...