Relationship Between The Effect Interrupting Mother-to-infant Transmission By Injected HBIG To Pregnant Women And Placenta Hepatitis B Virus Infection

ObjectiveTo study the relationship between the effect interrupting of HBsAg positive mothers injected HBIG and placenta hepatitis B virus infection, and to investigate the mechanism about the way of hepatitis B virus intrauterine infection. Methods92 HBsAg positive mothers were randomly divided into two groups,44 cases of study group were injected HBIG at 28,32,36weeks of pregnancy and 48 cases of control group were not injected. ELISA and PCR were used for detection of HBV serologic markers (HBV DNA and HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc ) of 92 HBsAg positive mothers prior to delivery and newborns of them before initiative and passive immunoprophylaxis.S-P immunohistochemical straining with monoclonal anti-HBs and anti-HBc antibodies was used for detection of HBsAg and HBcAg in decidual cell (DC),Trophoblastic cell(TC),villous stromal cell(VSC) and villous capillary endothelial cell(VCEC) of placentas born of 92 HBsAg positive mothers. Results1. The newborns intrauterine infection rate of 92 HBsAg positive mothers was 11.96%(11/92).The newborns intrauterine infection rate of HBeAg positive mothers was 21.15%, which was significantly higher than that of HBeAg negative mothers(0) ( X~2=7.71, P<0.05) . The newborns intrauterine infection rate of HBV DNA positive mothers was 18.64%,which was significantly higher than that of HBV DNA negative mothers(0) (X~2=5.33, P<0.05) .