Study On The Association Between Methionine Synthase (MS) Gene A2756G Polymorphism And Type 2 Diabetes Mellitus With Macrovascular Complications

Objective To explore the association between A2756G polymorphism of methionine synthase (MS) gene and type 2 diabetes mellitus with macrovascular complications. Methods The genotypes of MS gene A2756G polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique in 733 Chinese Han population of Anhui region including 178 normal subjects (CON), 273 diabetics without angiopathy (T2DM), 189 patients with diabetic macrovascular complications of atherosclerosis (T2DM+AS) [64 with coronary heart disease (T2DM+CHD), 111 with cerebral infarction (T2DM+CI) and 14 with diabetic food] and 93 non-diabetic patients with macroangiopathy of atherosclerosis (AS) [16 with coronary heart disease (CHD) and 77 with cerebral infarction (CI)]. We compared the genotype and allele frequencies in these groups and studied the relationship between the polymorphism and diabetic macrovascular complications by population-based association analysis. Results The G allele prevalences of MS gene A2756G polymorphism in Chinese Han population of Anhui region were significantly lower than that in western populations (P<0.05). There were no significant differences in the genotype and allele frequencies among these groups of CON,T2DM,T2DM+AS and AS (P>0.05). By general clinical characteristics age as well as systonic blood pressure (SBP) was significantly increased in T2DM+AS group or subgroups compared with T2DM (P<0.05). Logistic regression analysis showed that not the polymorphism but age increment or SBP was related independently and significantly to type 2 diabetes mellitus with macrovascular complications (OR=0.855, 95%CI=0.529~1.383, P=0.523 for G allele; OR=1.076,95%CI=1.028~1.127,P=0.002 for age; OR=1.028, 95%CI=1.002-1.054,P=0.033 for SBP). Conclusions The results suggested that age increment as well as SBP is an independent risk factor for type 2 diabetes mellitus withmacrovascular complications and the MS gene A2756G polymorphism has no relationship to the hereditary susceptibility for that.