Association Of Polymorphism Of Methionine Synthase Reductase Gene With Type 2 Diabetes Mellitus With Macrovascular Disease

Objective. To investigate association of methionine synthase reductase gene with type 2 diabetes mellitus with macrovascular disease (type 2 diabetes mellitus with coronary heart disease , type 2 diabetes mellitus with cerebral infarction , diabetic food). Methods.The genotypes of MTRR gene A66G polymorphism in 678 Chinese nan people in Anhui region, including 175 normal subjects, 240 type 2 diabetes mellitus, 176 type 2 diabetes mellitus patients with macrovascular disease (104 with cerebral infarction, 61 with coronary heart disease, diabetic food 11) and 87 patients with macrovascular disease only (13 with coronary heart disease and 74 with cerebral infarction), were determined by PCR-RFLP technique. We compared the genotypes frequency and allele frequency among these groups. Results We found that there were no significant differences of allelic frequencies of polymorphism A66G in MTRR gene among normal subjects group, 2-DM, 2-DM with macrovascular disease and macrovascular disease, but there were significant differences of genotype frequencies of polymorphism A66G in MTRR gene among them. The frequencies of genotype AA in type 2 diabetes mellitus and type 2 diabetes mellitus with macrovascular disease were lower than normal subjects, the group of macrovascular disease. The frequencies of genotype AG in type 2 diabetes mellitus and type 2 diabetes mellitus with macrovascular disease were higher than normal subjects, macrovascular disease. There were no significant differences of allelic frequencies of polymorphism A66G in MTRR gene among normal subjects group, 2-DM, 2-DM with coronary heart disease(2-DM with cerebral infarction), but there were significant differences of genotype frequencies, either. The frequencies of genotype AA in type 2 diabetes mellitus and type 2 diabetes mellitus with coronary heart disease(2-DM with cerebral infarction) were lower than normal subjects group.but the frequencies of genotype AG in type 2 diabetes mellitusand type 2 diabetes mellitus with coronary heart disease(2-DM withcerebral infarction)were higher than normal subjects.Conclusions These results imply MTRR A66G gene polymorphism is significantly associated with type 2 diabetes mellitus and type 2 diabetes mellitus with macrovascular disease .AG genotype may be a risk factor of type 2 diabetes mellitus and 2 type diabetes mellitus with macrovascular disease. AA genotype may be a protective factor of type 2 diabetes mellitus and type 2 diabetes mellitus with macrovascular disease. We did not found the association of the polymorphism with macrovascular disease, but further studies on larger populations are needed to prove these results.