Studies On Baicalin Accumulating Law In The Plant Of Scutellaria Baicalensis Georgi

To control and improve the quantity of the medicinal material by understanding baicalin accumulating law in the Scutellaria baicalensis georgi and external factors on accumulating baicalin, we studied baicalin distributing law in individual organs and the external factors to the content of baicalin such as temperature, light, fertilizer, and so forth. The content of baicalin during germinating of seeds was monitored by HPLC and HPCE. Individual tissues and segments of particular organs differ in their ability to accumulate secondary products. Therefore, particular organs, individual parts and different level radix were our studying objects. Moreover, PAGE was used to indicate the changes of POD in the different growth phases and individual organs of the plant. Histochemistry method was also used to determine baicalin content in different parts.Studies have shown that the baicalin is in every organ rather than in the seeds; the content of baicalin in radix is higher than that of flowers, then in leaves and then in stems; That in taproot is highest, in turn is sideroot, and fibreroot; The content in phloem is higher than that of xylem. The results have also demonstrated that baicalin begins to produce in the seventh day during germination of seeds and increases with the growth of young plant; Light accelerates the synthesis of baicalin, that is to say, the stronger the light is, the higher the content is. Besides light, temperature also has effects and 25C is the best temperature to the synthesis of baicalin; Fertilizer is also an important external factor to increase the content; the better fertilizer is one containing K+ and compound one containing N and P during germinating of seeds; Cu2+ restrains the baicalin synthesis of the plant cultured in sands, on the other hand, P and K elements increase the content of baicalin; sugars make different effects on different conditions; the content of protein and baicalin in linear correlation during germination of seeds; baicalin in the plant is in relationwith band 1 of peroxidase(POD).