Effect Of The Traditional Chinese Medical Astringent On Angiogensis And Expression Of MMP9 In Mice With S180 Sarcoma

Objective To investigate the effect on angiogensis as well as tumor growth of the traditional Chinese medical astringent, such as, Fructus Mume, Cortex Ailanthi, Cherokee Rose Fruit, Chinese Magnoliavine, Chinese Gall, Red Halloysite, Limonitum, and the mechanism of it.Method The chicken chorioallantoic membrane (CAM) angiogensis model was used to study the effect of these medical herbs on angiogensis. a model of transplanting tumor in mice was used to study the effect of these medical herbs on tumor growth. The expression of Vâ…¢-RAg and matrix metalloproteinase-9 (MMP-9) in tumor tissue were examined with immunohistochemical method. The microvessel density (MVD) of the tumour tissue was also measured with the Image Pro Plus medical image system. Result Cherokee Rose Fruit, Chinese Magnoliavine, Fructus Mume, Red Halloysite, Cortex Ailanthi can inhibit the angiogensis of CAM. Fructus Mume and Cortex Ailanthi can obviously inhibit the growth of sarcoma (S180) transplanted subcutaneously in mice (P<0. 05). There were fewer vessels in sarcoma tissue in Cortex Ailanthi group than in control group and CTX group(P<0.05). The expression of MMP-9 was lower in Cortex Ailanthi group and Fructus Mume group than in control group(P<0. 05). Conclusion Cortex Ailanthi can inhibit angiogensis in CAM and tumor tissue, and can also suppress the growth of sarcoma (S180) transplanted in mice and the expression of MMP-9 in the sarcoma tissue.