| Backgroud:Chronic ulcerative colitis is such a kind of disease with the essential character of colitis and proctitis, but its etiology is still unclear. The clinical symptoms include bellyache,diarrhea, bloody purulent stool, and varying degrees of systemic symptoms, with a character that remission and recurrence appears alternately. Because of its heterogeneity, high recurrence rate, long course as well as low cure rate,World Health Organization has listed it as one of the refractory diseases. So far, its etiology and pathogenesis is unclear. And it is considered to be the consequence of the interaction of multiple factors including immunity, infection, genetic, environmental, and spiritual factors. The Western medicine classic treatment is still based on the SA-SP or5-ASA aminosalicylate preparationsmain. What’s more, severe patients were treated combined with hormone and immunosuppressive medicine, but there is still no radical cure now. Recently, Traditional Chinese Medicine scholars have being done lots of studies on the treatment of UC with Chinese medicine,According to their findings, traditional Chinese medicine supplied multi-target role for the treatment of UC, So there are broad foregrounds of application of traditional Chinese medicine.Chronic ulcerative colitis belongs to the category of "diarrhea" and "dysentery" in Traditional Chinese medicine. Traditional Chinese medicine suggested that, the happen of diarrhea and dysentery is due to malfunction of spleen and stomach, and closely related with large intestine loses normal transmission and digest function, liver-qi can’t dredge smoothly and the insufficiency of kidney-yang, lose contro-lling actions. Astrigent and antidiarrheal is mainly composed with Chinese herble medicine which has a warm-natured and solid astringent character, mainly treasure the syndrome that long diarrhea belongs to spleen-kidney deficiency-cold, less evil and more zang-fu deficiency. Rencently, Traditional Chinese medicine studys around astrigent and antidiarrheal treasuring UC suggest this method has valid effect. While there is few study about it’s cure mechanism for UC. So, the experiment succeeded in establishing chronic ulcerative colitis rat model first, with the purpose to discuss mechanism of astrigent and antidiarrheal,we observe the opsonification of astrigent and antidiarrheal on gut hormone, cytokine and colonic mucosa repair, All of our study will provide the scieniific and reliable evidence for clinica lapplication.Methods:1. To generate Chronic UC models with rats, observing their signs and symptoms as well as colon pathological changes.2. Observing the variation of signs and symptoms, colon pathological changes and testing gut hormone levels in colon.3. Detecting levels of Tumor necrosis factor-a and Interleukin-10in serum4.Detecting content of colonic transforming growth factor-β1and vascular endothel-ial growth factor.Results:1. After the first administration of TNBS, we dosed DSS during the remission DAI score showed a relief-rising-relief trend. Both the CMDI and DAI of model group were significantly higher than that of normal control group. Ulcers and inflammation was still visible in the21st day at colon tissue in the test group.2. Astrigent and antidiarrheal can reduce the expression of chronic ulcerative of colon tissue substance P, DAI scores and CMDI scores, significantly different was found between groups. After administration, newborn colonic mucosa and glands was obser-ved in high and middle dose group. The area of ulcer reduced along with inflamemat-ory response mitigation.3. Expression of TNF-a in serum was significantly inhibited while interleukin-10was promoted4. Both colon tissue transforming growth factor-β1and vascular endothelial growth factor’s content in model group and test group were higher than control group. High-dose and middle-dose were significantly lower than model group at the end of experiment. Results from immunohistochemistry indicated that astrigent and antidiarrheal can significantly promote the expression of colon tissue vascular endothelial growth factor.Conclusion:1. Using TNBS to generate fundamental inflammation changes and giving intermitte-nt stimulation with DSS during remission can simulate human chronic ulcerative colitis featuring in remission and recurrence alternately happen. The histopatholo-gyical change was similar to human.2. Astrigent and antidiarrheal can cure diarrheas and hematochezia promoting the repairment of colonic mucosa tissue. Reducing the expression of substance P may be the one of the therapeutic mechanism.3. Regulating immune function may be one way of astrigent and antidiarrheal in reducing inflammation degree.4. Expression of transforming growth factor-(31involved in the UC, indicate a potential application in reflecting degrees of colon injury and disease activity degree. Astrigent and antidiarrheal can promote colon injury hearing by means of enhancing the expression of vascular endothelial growth factor.5. Astrigent and antidiarrheal supply multiple target treatment in UC, related with anti inflammatory, regulating colon gastrointestinal hormones, improving local microci-rculation and promoting colon injury healing. |
PDF Full Text Request