Relation Of Hepatitis B Virus Genotype And The Disease Severity Of Chronic Hepatitis B , Efficacy Of Interferon-α

Paitents with chronic hepatitis B in Nanfang hospital who were treated with interferon-a were surveyed and the infected virus genotypes were tested. The demography, Alanine aminotransferase(ALT), HBV DNA level and liver biopsy of them were collected and analysed. Thus the relation of genotype with severity of disease and interferon response could be observed.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to identify the genotypes of HBV. Patients of Nanfang Hospital during 1998.1~2002.4 were investigated and their demography, ALT, HBV DNA and liver biopsy were studied. In total 130 patients, 70 was genotype B and 60 was genotype C. There was no significant difference between genotype B and C in the following aspects: age(t =-1.407, P=0.162), sex( x 2= 1.327, P=0.249), ALT(U=l955.000, /M).468), HBV DNA(U=1991.500, P=0.589), liver inflammation necrosis index(U= 1922.000, P=0.373) and liver fibrosis index (U=1950.500, P=0.467). This implyed genotype B and C have no relation to clinic disease severity and the most possible reason was genotype B and C shared the same preC/C domain, producing the same HBeAg and so stimulated the same T lymphocyte immunity activity.Patients with CHB in studied cohort were followed up. The relation of genotype and efficacy of interferon-a were assessed. Genotype B and C had no significant difference in biochemical response ( x 2=0.007, P=0.932), virological response( x 2=0.050, P=0.823) and sustained response( x2=0.201, P=0.654). This implyed genotype could not be a prognosis index.