The Analysis Of Bacterial Culture And Flora From Bronchoaleolar Fluid In Seawater Drowning Dogs

Objective To investigate the changes of bacterial culture and flora dogs bronchus pulmonary alveolar fluid in seawater drowning dogs Methods 10 mongrel dogs were randomly divided into two groups:seawater investigation group A group (5 dogs) and fresh water investigation group B group (5dogs), seawater investigation and fresh water investigation were carried out by following Modell method. Under aseptic condition extracted of pulmonary alveolus fluids for bacterial culture and to takeing of body temperatures. Through bronchoscope immediately and at 4h,8h,12h,24h,36h,48h time points. Lung tissues were takes for bacterial quantity culture at 48h after drowning.Results The bronchus pulmonary alveolar fluids immediately afterseawater drowning in group A showed negative in colture or only a small quantity of bacterial strain . the bacterial strain had to restrain after 4h, at each pead points. The bacterial strain in A group was mainly gram negative bacillus in accounted for 56%, gram positive 39%, and fungus 5%. The B groups bacterial quantity after 8h were lower than A group. The bacterial strain had no difference as the A group, Gram negative bacteria for 58%, Gram positive bacteria 40%, and fungus 2%. The ocean special bacteria group were not founded in these experimentation culture. The lung tissue bacterial quantitative analysis in group A were over the those bacterial infection index ( 5*105 ) and was not attained in the B group. The bodytemperature in A group was higherthan B group after 8h and reached to 40 C at 48h.Conclusion The lung injuries are more serious after sea water drowning,last for longer time and creates a condition for bacterial infection. There for the active prevent and cur the pulmonary infective is the keep point not to be overlooked.