A Study On Bβ-Fibrinogen-148C/T Polymorphism And Platelet Membrane Glycoprotein VI T13254C Polymorphism Of Coronary Heart Disease

Objective To investigate the function of platelet membrane glycoprotein VI(GPVI) T13254C polymorphism and B β -fibrinogen -148C/T polymorphism in arterial thrombosis and the association with coronary heart disease.Methods In a case-control study the fibrinogen levels were detected by Clauss in 179 subjects with angiographically documented coronary artery disease and 164 subjects without CHD (CHD was excluded by coronarography). Restriction fragment length polymorphism (RFLP) analysis was performed to screen the GPVI T13254C polymorphism and B β -fibrinogen -148C/T polymorphism.Results (1) Mean plasma Fib levels were significantly higher in patients with coronary heart disease than that in controls (3.34?.58g/l vs 2.70?.57g/l, p<0.01).There was a strong correlation between plasma Fib levels and coronary artery stenosis score (r=0.437, P<0.01). Logistic regression analysis showed that plasma Fib levels correlated with the risk of CHD. After ajustment for the risk factors, Fib was still an independent risk factor of CHD(r=0.386, P<0.01). (2)Patients carrying the 148 T alleles showed higher plasma Fib levels compared with those carrying 148CC genotype in CHD group, (P<0.01). Plasma Fib levels was significantly associated with -148C/T polymorphism among smokers in CHD group, ( PO.01) (3) No significant difference was found in the frequencies of B β -fibrinogen -148C/T genotype and alleles between controls and CHD patients4(x 2=1.86, P>0.05; x 2=1.57, P>0.05 respectively). (4) The frequencies of GPVI T13254C C alleles is 0.026 in all of subjects. No significant differences was found in the frequencies of GPVI T13254C genotype and alleles between controls and CHD patients (x 2=0.036, P>0.05; x 2=0.045, P>0.05 respectively). (5) No significant differences was found in mean plasma Fib levels between GPVI T13254C TT genotype and CT genotype.Conclusion (1) The level of fibrinogen was an independent risk factor of CHD. There was strong relationship between fibrinogen and the severity of CHD. (2) There was obvious relationship .between theB -fibrinogen -148C/T mutation and high fibrinogen level in CHD group. Smokers carrying the 148 T alleles showed higher plasma Fib levels compared with those carrying 148CC genotype in CHD group, (P<0.01). (3). The B -fibrinogen -148C/T polymorphism was not correlated with the risk of CHD. (4) In this study the rate of GPVI T13254C mutation was quite low, GPVI T13254C polymorphism was not associated with an increased risk of CHD. (5) There was no association between the GPVI T13254C mutation and fibrinogen level.